枳和枳橙的茎尖培养与增殖研究

A Study on Shoot Tip Culture and Inducing Proliferation of Trifoliate Orange and Citrange

采用枳(实生树)和枳橙(嫁接树)的茎央在MS+Kt2.0mg/L+NAA0.1mg/L+蛋白胨300 mg/L+GA_30.2mg/L培养基上具有良好的分化效果,可诱导大量丛生芽,分化出根、茎、叶俱全的完整植株.Kt能克服顶端优势,促进腋芽萌发,对于茎尖繁殖的最适浓度为2.0mg/L.适量的蛋白陈可促进茎尖抽枝成苗.用100ppmIBA浸泡新梢基部4—6h,有利于试管内生根.200ppm ABT生根粉和100 ppmIBA处理无根试管苗,成功地在试管外诱导生根,简化了试管苗繁殖的步骤.

This article deals with a promising effect on differentiation of the shoot apex of Trifoliate orange seedling and grafted citrange where it could indnce numerous clumped buds and develop into an plant with root, stem and leaf when it was cultivated in a MS in addition to Kt 2.0 mg/1, NAA0.1mg/l, Peptone 300mg/l and GA30.2mg/l, A test of Kt concentrations shows that Kt could inhibit the apical dominance and promote the germination of the axillary bud. The optimum concentration of Kt for the apex proliferation is 2.0mg/l. An appropriate amount of peptone could help an apex inducing shoot and eventually form seedlings. A treatment of the base of a young shoot by soaking in 100 ppm 1 BA solution for se veral hours is helpful to root formation in vitro. Treated with 200 ppm ABT or 100 ppm IBA, a rootless shoot in vitro could successfully induce roots outside the tube, thus the propagation in vitro could be simplified considerably.