不同海拔地区实验动物肝脏G—6—P酶的电镜观察

ELECTRON MIGROSCOPIC OBSERVATION OF G-6-Pase ON LIVER CELLS OF EXPERIMENTAL ANIMAL AT DIFFERENT ALTITUDES

本文报道了平原兔、狗及藏系羊肝细胞G-6-Pase活性的电镜观察。把生活在平原地区(南京海拔20m)的16只兔和14只狗分成三组:(1)留在平原地区的实验动物处死后经观察,G-6-Pase是定位在肝细胞核外膜及内质网上。(2)被带到高原(西宁2 260m和大武3 800m)的兔和狗第二天被处死,经观察G-6-Pasd全部被抑制。(3)被带到高原大武地区的兔和狗第七天被处死,则见G-6-Pase得到恢复。高原藏系羊下到平原江苏、苏州第十日被处死以及由苏州再带回高原西宁第七天被处死,G-6-Pase均未得到恢复。作者讨论了这些改变的可能意义。

This article selected the healthy 16 rabbits that lived in plain (NanKing,20m above sea level)and the average weight is 2.5kg,healty 12 dogs that have a light wool and the average weight is 20.5kg.In the high altitude(Tian Jun,3 800m),we adopted 4 Tibetan sheeps averaging weight 30.5kg,in the DaWu area(3 800m adove sea level)4 dogs that dorn and lived in there,and average weight is 22.Okg was studied.All of the experimental animals were divided into five groupa;A group,the rabbits and dogs that born and lived in plain(control group);B group,the rabbits which lived in plain entered directly the Xining (2260m above sea level)and were killed at the next morning;C_1 group,the animals lived in plain and entered Dawuarea (3 800m)in short time,the group animals was divided into two small groups further: cl group were killed in next morning after arriving,C2 group were killed on the 7th day moring;D group was the dogs that born and lived in the high alti- tude (Dewu,3 800m);E group animals were the altitude Tiantan sheeps that were sent to the plain from TlanJun and killed in a month;further El group was which the altitude sheeps lived in the plain 45 days and then weresent back to the Xining.There was two female animals and two males'in each group.The transmission electron microscope was used to observe the G6Pase activity change of the liver cells in all of the exerimantal animals.