口腔鳞癌患者染色体畸变和脆性部位的研究

CHROMOSOME ABERRATION AND FRAGILE SITES IN LYMPHOCYTES FROM PATIENTS WITH ORAL SQUAMOUS CELL CARCINOMA

本报道TC199培养基加阿糖胞苷诱导10名口腔鳞癌患者外周血经PHA刺激的淋巴细胞染色体畸变和脆性部位表达。结果显示,口腔癌患者自发性和诱导性染色体畸变率显著高于正常人,经阿糖胞苷诱导后,癌患者脆性部位表达率高于正常人,表达范围比正常人广,脆性部位与癌断裂点及癌基因座位相关。

This paper reports the fragile sites (FS) induced by l-β-D-arabinofuranosylcytosine (Ara-C) in lymphocyte chromosomes from 10 patients with oral squamous call carcinoma and from 10 healthy donors.Peripheral blood lymphocytes were cultured in TC 199 medium containing 5% calf bovine serum and phytohemagglutinin.The results were as following: spontaneous and induced chromosome aberration rates in lymphocytes from patients were significantly higher than those from healthy donors; FS induced by Ara-C in lymphocytes from patients and donors were 21 and 10 resectively; frequency of FS expression det-ected in patients was significantly higher than that in donors; and statistically, FS correlated with cancer breakpoints and oncogenes.