紫杉醇联合金雀异黄素用于肺癌细胞的抑制效果评估

Evaluating the effect of paclitaxel and genistein on lung cancer A549 cell

目的探讨紫杉醇(paclitaxel,PTX)联合金雀异黄素对肺癌细胞的抑制作用。方法将肺癌A549细胞随机分为生理盐水组、紫杉醇干预组、金雀异黄素干预组和联合干预组。MTT法研究紫杉醇联合金雀异黄素对肺癌A549细胞的增殖抑制情况。流式细胞术检测给药后肿瘤细胞凋亡情况。建立A549肺癌裸鼠移植瘤模型,计算抑瘤率。结果肺癌A549细胞给药48h后,紫杉醇干预组、金雀异黄素干预组和联合干预组对肿瘤细胞的抑制率分别为(57.0±4.5)%、(34.0±4.6)%和(77.0±4.4)%,差异具有统计学意义(P<0.01);紫杉醇干预组、金雀异黄素干预组和联合干预组凋亡率分别为63.4%、41.2%和84.5%,而生理盐水组凋亡率为7.6%,差异具有统计学意义(P<0.01);荷瘤小鼠实验结果显示,相比于生理盐水组,金雀异黄素组、紫杉醇组和联合干预组对肿瘤生长的抑制率分别为(18.2±3.4)%、(32.1±4.2)%和(63.4±5.1)%,联合干预组与其他单独给药组间差异具有统计学意义(P<0.01)。结论紫杉醇与金雀异黄素均具有抑制肺癌细胞增殖的作用,2者联用能够发挥协同作用,增强抗肿瘤能力。

Objective To evaluate the effect of paclitaxel and genistein on the treatment of lung cancer. Methods A549 cells were divided into saline group, PTX group, Gen group and(PTX+Gen) group. The anti-proliferation efficiency of paclitaxel combined with genistein was evaluated by MTT assay and flow cytometry test. A 549 cells were injected into mice by hypodermic in order to establish the animal model, which were used to evaluate the effect of anti-tumor. Results The cell viability of A 549 cells were(57.0±4.5)%,(34.0±4.6)% and(77.0±4.4)% after the treatment of paclitaxel, genistein and paclitaxel+ genistein, respectively(P<0.01). The effect of saline induced cells apoptotic was 7.6%,while paclitaxel, genistein and paclitaxel + genistein were 63.4%、41.2% and 84.5%, respectively(P<0.01).The tumor inhibitory rates were(18.2±3.4)%,(32.1±4.2)% and(63.4±5.1)% in vivo for genistein, paclitaxel and paclitaxel+ genistein(P<0.01). Conclusion Both paclitaxel and genistein can inhibit the growth of lung cancer cell lines, and the combination of paclitaxel and genistein may be a potentially effective treatment for lung cancer.