大豆黄酮对人肝癌SMMC7721细胞增殖及肿瘤干细胞CD133表达的影响

Effect of daidzein on hepatocellular carcinoma SMMC7721 cell proliferation and tumor stem cell CD133 expression

目的探讨大豆黄酮对人肝癌SMMC7721细胞增殖及肿瘤干细胞CD133表达的影响。方法将肝癌SMMC7721细胞分成6组:阴性对照组不加药;根据添加大豆黄酮浓度的不同,分成100μg/mL组、200μg/mL组、300μg/mL组、400μg/mL组、500μg/mL组,培养24 h、48 h、72h后MTT法检测细胞的抑制率、测定每组细胞中己糖激酶和碱性磷酸酶活性和CD133表达水平并进行比较。结果大豆黄酮浓度越高,对肝癌细胞的抑制作用越强;随着处理时间的延长,抑制作用有所下降;尤其在400μg/mL浓度组和500μg/mL浓度组,时间效应更明显。与对照组相比,经不同浓度大豆黄酮处理的肝癌细胞已糖激酶活性和碱性磷酸酶活性均显著降低(P<0.01),肿瘤干细胞CD133表达明显下降(P<0.01),并且浓度越高下降幅度越大。结论大豆黄酮能够抑制肝癌SMMC7721细胞的增殖,并降低肿瘤干细胞CD133的表达。

Objective To discuss the effect of daidzein on hepatocellular carcinoma SMMC7721 cell proliferation CD133 expression on tumor stem cell.Methods Hepatocellular carcinoma SMMC7721 cells were cultured,digested and passaged,and divided into six groups with different drug:control group with no daidzein,100 μg/mL daidzein group,200μg/mL daidzein group,300μg/mL daidzein group,400μg/mL daidzein group ,500μg/mL daidzein.The inhibition ratio,hexokinase,alkaline phosphatase and CD133 levels in SMMC7721 cell were detected and compared at 24 h,48 h,72 h among those groups. Results The inhibition ratio was increased by Daidzein dose increasing,and decreased apparently by times extending,especially in 400μg/mL and 500μg/mL daidzeingroups.Compared with control group,the hexokinase and alkaline phosphatase activity and CD133 expression were decreased apparently in groups treated with daidzein(P<0.01).The more the dose,the higher the drop(P<0.01).Conclusion Daidzein can inhibit hepatocellular carcinoma SMMC7721 cell proliferation,and inhibit CD133 expression on tumor stem cell.