抗血管内皮细胞抗体在大鼠肺气肿中的作用及其与甲强龙干预的关系研究

Effects of anti-endothelial cell antibody(AECA)and intervention of methylprednisolone in emphysema rats

目的观察抗血管内皮细胞抗体(anti-endothelial cells antibodies,AECA)在吸烟诱导的肺气肿中的作用,以及甲强龙的干预影响。方法 39只大鼠随机分为正常组、模型组及甲强龙干预组(n=13)。模型组及干预组进行被动吸烟,烟雾暴露1个月时,对干预组皮下注射甲强龙(10 mg/kg,1次/d),每周6 d;烟雾暴露90 d后,抽取下腔静脉血,处死大鼠,同时收集支气管肺泡灌洗液(bronchial alveolar lavage fluid,BALF);检测各组大鼠血清、支气管肺泡灌洗液中的AECA。肺组织切片苏木素伊红染色后观察组织形态学改变,测定肺平均内村间隔(mean linear intercept,MLI)、平均肺泡数(mean alveolar numbers,MAN)。结果与正常组、干预组比较,模型组血清及支气管肺泡灌洗液中的AECA、MLI升高(P<0.05),但MAN显著降低(P<0.05);肺泡灌洗液AECA与MLI正相关(r=0.821,P<0.05),与MAN负相关(r=-0.894,P<0.05)。结论 AECA可能参与吸烟诱导的肺气肿形成,并可能与其严重程度相关;甲强龙可能通过降低AECA含量影响肺气肿形成。

Objective To explore the effects and mechanisms of anti-endothelial cell antibody (AECA )in the alveolar cell apoptosis of the emphysema rats induced by smoking,and to discuss the intervention effects of methylprednisolone.Methods 39 male Sprague-Dawley rats were randomly divided into normal control group,model group,and intervention group,with 13 rats in each group.Emphysema models were established in the latter two groups.After exposing to cigarette smoking for one month,methylprednisolone injected intraperitoneally in the intervention group(10 mg/kg,1 time/d),6d/week.On the 90th day,inferiora vena cave blood samples were collected and all rats were sacrificed.The levels of AECA were detected in bronchial alveolar lavage fluid (BALF )and serum,respectively.Pathological changes were observed in lung tissues stained by hematoxylin eosin, quantitative determination of lung average mean linear intercept(MLI)and mean alveolar number(MAN)were preformed. Results Compared with normal group and intervention group,the levels of AECA in BALF and serum,MLI in the model group were higher(P<0.05 ),but the levels of MAN was lower (P<0.05);There is a positive correlation between AECA in BALF and MLI of rats(r=0.821,P<0.05),a negative correlation between AECA in BALF and MAN(r=-0.894,P<0.05.Conclusions ACEA may enroll the pathogenesis of emphysema in rats induced by smoking and related with the severity.Methylprednisolone may inhibit the formation of emphysema by reducing the expression level of AECA in airway.