虫草菌液对糖尿病肾病肾小管上皮细胞转分化的影响

Effect of Cordyceps sinensis liquid on diabetic nephropathy renal tubular epithelial cell transdifferentiation

目的探讨虫草菌液对糖尿病肾病肾小管上皮细胞转分化的影响。方法将不同浓度的虫草菌液和高糖作用于人近端肾小管上皮细胞,根据加入剂量和组份不同分成以下5组:对照组给予5.5 mmol/L D-葡萄糖;高糖组给予30 mmol/L D-葡萄糖;实验组1给予30 mmol/L D-葡萄糖+5μg/mL虫草菌液;实验组2给予30 mmol/L D-葡萄糖+10μg/mL虫草菌液;实验组3给予30 mmol/L D-葡萄糖+20μg/mL虫草菌液。检测各组细胞内E-钙粘蛋白(E-Cadherin),纤连蛋白(fibrous protein,FN),α-平滑肌肌动蛋白(a-smooth muscle actin,α-SMA)及整合素连接激酶(Integrin Linked Kinase,ILK)蛋白的表达。结果与对照组相比,高糖组E-cadherin蛋白表达显著降低(P<0.01),且随着虫草菌液加入的量升高,E-cadherin蛋白表达也逐渐升高(均P<0.01)。与对照组相比,高糖组α-SMA蛋白和FN蛋白表达均显著升高(P<0.01),且2蛋白在实验组2和实验组3中表达均较高糖组显著下降(均P<0.01)。与对照组相比,高糖组ILK蛋白表达显著升高(P<0.01),实验组3 ILK蛋白表达较高糖组显著下降(P<0.01)。结论虫草菌液能够抑制肾小管上皮细胞向肌成纤维细胞的转化,从而抑制肾小管间质纤维化。

Objective To discuss the effect of Cordyceps sinensis liquid on Diabetic Nephropathy renal tubular epithelial cell transdifferentiation.Methods Diabetic Nephropathy renal tubular epithelial cells were treated with different dose of Cordyceps sinensis liquid and high glucose,and divided into five groups.Control group was added 5.5 mmol/L D-glucose,High glucose group was added 30 mmol/L D-glucose, Experimental group 1 was added 30 mmol/L D-glucose and 5μg/mL Cordyceps sinensis liquid,Experimental group 2 was added 30 mmol/L D-glucose and 10 μg/mL Cordyceps sinensis liquid,Experimental group 3 was added 30 mmol/L D-glucose and 20 μg/mL Cordyceps sinensis liquid.The expression of E-cadherin protein,FN protein,α-SMA protein and ILK protein in each group were detected by western blot. Results Compared with control group,the expression of E-cadherin protein in high glucose was decreased significantly(P<0.01),and with the addition of Cordyceps bacteria increases,E-cadherin protein expression in three Experiment group were gradually increased(P<0.01).Compared with control group,the expression ofα-SMA protein and FN protein in High glucose group were increased significantly (P <0.01 ),and both two protein in Experiment group 2 and Experiment 3 were significantly lower than High glucose group(P<0.01).Compared with control group,the expression of ILK protein in high glucose group was significantly higher(P<0.01 ),and its expression in Experiment group 3 was significantly lower than High glucose group (P<0.01 ). Conclusion Cordyceps sinensis liquid can inhibit diabetic nephropathy renal tubular epithelial cell translating into myofibroblast,then inhibit renal tubule interstitial fibrosis.