摘要
Objective:To evaluale physico-chemical properties and antimicrobial potential of indigenous honey samples against different reference strains including Escherichia coli ATCC 8739.Enterobacter aerogenes ATCC 13048.Pseudomonas aeruginosa ATCC 9027.Bacillus subtilis ATCC6633.Staphylococcus aureus ATCC 25923.Salmonella typhi ATCC 14028,Klebsiella pneumonia ATCC 13883.Aspergillus niger ATCC 16404.Rhizopus oligasparus PCSIR1.Candida albicans ATCC14053 and Candida utilis ATCC 9950.Methods:By using standard methods samples were evaluated for their antimicrobial properties including additive effect of starch and non—peroxidase activity,antioxidative properties(phenol contents,flavonoid contents,1,1-diphenyl-2-pierylhydrazyl radical scavenging activity).Prior to this evaluation,complete physico-chemical properties including pH,color,ash contents,protein contents,moisture contents,hydroxymethyl furfural contents,total sugar contents,reducing sugar and non-reducing sugar contents were analyzed.Results:Relatively higher ash contents were found in the Siddar honey i.e.(0.5900±0.0336)%and small honey showed relatively higher protein contents i.e.(777.598±9.880) mg/kg.The moisture contents of tested honey samples ranged between 13.8%—16,6%,total sugar contents foam 61.672%-72.420%and non-reducing sugar contents from 1.95%—3.93%.Presences of phenolic contents indicate higher antioxidant potential of these honey samples.All bacteria showed clear inhibition zones in response to tested honey samples whereas fungi and yeast showed inhibition at higher concentrations of these honey samples.For Escherichia coli.Bacillus subtilis.Salmonella typhi.Pseudomonas aeruginosa and Aspergillus niger,overall the small honey showed the higher activity than other honey samples.Conclusion:Physico-chemical analysis of honey samples confirmed good quality of honey according to the standards set by European Union Commission and Codex Alimcntarius Commission.Evaluation of these honey samples confirms antimicrobial potential of particular types of honeys indigenous to Pakistan.
Objective:To evaluale physico-chemical properties and antimicrobial potential of indigenous honey samples against different reference strains including Escherichia coli ATCC 8739.Enterobacter aerogenes ATCC 13048.Pseudomonas aeruginosa ATCC 9027.Bacillus subtilis ATCC6633.Staphylococcus aureus ATCC 25923.Salmonella typhi ATCC 14028,Klebsiella pneumonia ATCC 13883.Aspergillus niger ATCC 16404.Rhizopus oligasparus PCSIR1.Candida albicans ATCC14053 and Candida utilis ATCC 9950.Methods:By using standard methods samples were evaluated for their antimicrobial properties including additive effect of starch and non—peroxidase activity,antioxidative properties(phenol contents,flavonoid contents,1,1-diphenyl-2-pierylhydrazyl radical scavenging activity).Prior to this evaluation,complete physico-chemical properties including pH,color,ash contents,protein contents,moisture contents,hydroxymethyl furfural contents,total sugar contents,reducing sugar and non-reducing sugar contents were analyzed.Results:Relatively higher ash contents were found in the Siddar honey i.e.(0.5900±0.0336)%and small honey showed relatively higher protein contents i.e.(777.598±9.880) mg/kg.The moisture contents of tested honey samples ranged between 13.8%—16,6%,total sugar contents foam 61.672%-72.420%and non-reducing sugar contents from 1.95%—3.93%.Presences of phenolic contents indicate higher antioxidant potential of these honey samples.All bacteria showed clear inhibition zones in response to tested honey samples whereas fungi and yeast showed inhibition at higher concentrations of these honey samples.For Escherichia coli.Bacillus subtilis.Salmonella typhi.Pseudomonas aeruginosa and Aspergillus niger,overall the small honey showed the higher activity than other honey samples.Conclusion:Physico-chemical analysis of honey samples confirmed good quality of honey according to the standards set by European Union Commission and Codex Alimcntarius Commission.Evaluation of these honey samples confirms antimicrobial potential of particular types of honeys indigenous to Pakistan.
基金
Supported partly by Higher Education Commission Pakistan,(via IPFP Grant No.3782)
