siRNA干扰胃癌细胞内源性VEGF-C的基因和蛋白表达的实验研究

The Study of Interfering of Endogenous VEGF-C Genes and the Protein Expression Gastric Cancer Cell with siRNA Technique

目的探索siRNA干扰技术阻断胃癌细胞内源性血管内皮生长因子-C(VEGF-C)的基因和蛋白表达的有效性。方法培养胃癌细胞株SGC7901,选择GenBank的Accession No.BC035212.1设计5条VEGF-C基因特异的siRNA引物,并进行同源性检索及合成。分别观察不同siRNA引物转染胃癌细胞株SGC7901后对其内源性VEGF-C mRNA和蛋白表达的影响。结果本实验成功将VEFG-C-siRNA导入胃癌细胞,5条siRNA都能抑制VEGF-C mRNA及蛋白的表达,但siRNA引物3、4阻断表达的效果最佳。结论 siRNA技术可以有效地阻断胃癌细胞中内源性VEGF-C的基因及蛋白表达。

Objective To explore the interfering effects of siRNA on endogenous VEGF-C genes and the protein expression in gastric cancer cells.Methods Cultured gastric cancer cell line SGC7901 cells were prepared in vitro.Five siRNA primers of VEGF-C were designed depending on the sequence of Accession No.BC035212.1in Genbank.After homology analysis,the primers were synthesized and transfected into SGC7901 cells.The endogenous VEGF-C mRNA level and its protein expression were observed.Results VEGF-C-siRNA was inserted into the gastric cancer cell successfully.Five siRNA primers of VEGF-Ccould inhibit VEGF-C genes and protein expression.Conclusion siRNA could block the endogenous VEGF-C genes and the protein expression in gastric cancer cell.