枸杞多糖对糖尿病大鼠血糖水平及机体免疫功能的影响

Influence of Lycium Barbarum Polysaccharide on Blood Glucose and Immune Function of Diabetic Rats

目的探讨枸杞多糖对糖尿病大鼠血糖水平及机体免疫功能的影响。方法从70只Wister雄性大鼠中随机选取12只为正常对照组,其余大鼠尾静脉注射链脲佐菌素制备1型糖尿病大鼠模型(造模组)。造模第7天将血糖水平>11.1 mmol/L的48只大鼠随机分为模型组、多糖高剂量组、多糖中剂量组和多糖低剂量组,各12只。经干预实验,比较各组大鼠血糖水平、脾脏指数、胸腺指数、脾淋巴细胞刺激指数及外周血T淋巴细胞亚群。结果造模初始造模组血糖水平较正常对照组升高,差异有统计学意义(P<0.01),说明造模成功。(1)第2周和第4周多糖组血糖水平均较模型组降低(P<0.01),但多糖组两两比较,差异无统计学意义(P>0.05)。(2)多糖组的胸腺指数和脾淋巴细胞刺激指数均较模型组升高,差异有统计学意义(P<0.01)。(3)多糖组的CD3、CD4及CD4/CD8值较模型组升高,多糖组的CD8值较模型组降低,差异有统计学意义(P<0.05)。结论枸杞多糖具有较好的调节血糖水平的作用,其降血糖作用可能与改善糖尿病大鼠免疫功能低下有关。

Objective To observe the influence of lycium barbarum polysaccharide on blood glucose and immune function of diabetic rats.Methods 12 Wister rats were selected from 70 as the control group.The other rats were prepared for type 1 diabetes mellitus after receiving intravenous injection of STZ.After seven days,48 rats with blood glucose > 11.1 mmol / L were randomly divided into model group,polysaccharide high dose group,middle dose goup and low dose group with each 12 rats.After intervention experiment,the blood glucose,spleen index,thymus index,spleen lymphocyte conversion rate and peripheral blood T lymphocyte were compared between each group.Results At the beginning of modeling,the rats used for the modeling showed significantly higher blood glucose(P < 0.01),indicating successful modeling.(1) Compared with the model group,the blood glucose in polysaccharide group at week 2 and week 4 was reduced,but there was no significant difference between polysaccharide groups(P > 0.05).(2) Thymus index and spleen lymphocyte stimulation index in polysaccharide group were significantly higher than the model group(P < 0.01).(3) The CD 3,CD 4 and CD 4 / CD 8 in polysaccharide group were significantly higher than the model group,the CD 8 in polysaccharide group was significantly lower than the model group(P < 0.05).Conclusion Lycium barbarum polysaccharide can reduce blood glucose level,and it may be due to the improvement of immune function of diabetic rats.