胃腺癌组织中p53和NF-κBp65蛋白表达的关系及意义

THE SIGNIFICANCE OF P53 AND NF-KBP65 PROTEIN EXPRESSION AND THEIR RELATIONSHIP IN GASTRIC ADENOCARCINOMA TISSUES

目的:探讨p53和NF-κBp65蛋白在胃腺癌中表达及与临床病理特征的关系。方法:采用免疫组化S-P法检测100例胃腺癌及42例胃腺癌淋巴结转移灶中p53和NF-kBp65蛋白表达水平。结果:胃腺癌组织中p53阳性表达率为53%。低分化胃腺癌组的阳性表达率明显高于中高分化胃腺癌组的阳性表达率(χ2=7.486,P<0.01);在有淋巴结转移的胃腺癌组中阳性的表达率明显高于无淋巴结转移的胃腺癌组(χ2=6.368,P<0.05);Ⅲ、Ⅳ期胃腺癌组的阳性表达率明显高于Ⅰ、Ⅱ期组的阳性表达率(χ2=13.864,P<0.01);胃底/贲门、胃体、胃窦/幽门胃腺癌之间的阳性表达率无显著差异性(χ2=2.529,P>0.05)。胃腺癌组织中NF-κBp65阳性表达率为58%。低分化、中高分化胃腺癌之间,有淋巴结转移、无淋巴结转移胃腺癌之间,Ⅲ、Ⅳ期和Ⅰ、Ⅱ期胃腺癌之间的阳性表达率均存在显著的差异性(χ2=4.624,P<0.05;χ2=3.876,P<0.05;χ2=7.436,P<0.01),而胃腺癌部位阳性表达率无显著差异性(χ2=3.306,P>0.05)。42例淋巴结转移灶中p53和NF-kBp65阳性表达率分别为30.95%和35.71%,均明显低于胃腺癌原发灶(χ2=4.861,P<0.05;χ2=8.052,P<0.05),p53和NF-kBp65在胃腺癌原发灶和淋巴结转移灶中的表达均呈正相关(c=0.505,P=0.001;c=0.316,P=0.041)。结论:p53的异常表达和NF-κBp65分别在胃腺癌的发展、侵袭和转移中起到重要作用;p53的和NF-κBp65在胃腺癌中协同表达,提示可能共同参与胃腺癌的增殖、侵袭和转移;联合检测胃腺癌中p53和NF-κBp65表达,可为临床诊断病情发展程度提供指导意义。

Objective:To explore the protein expression levels of p53 and NF-kBp65 in the tissues of Gastric adenocarcinoma, and their relationship with patients'' clinical and pathological features. Methods:Immunohistological S-P staining was used to detect p53 and NF-kBp65 protein expression in 100 cases of primary tumor tissues,and in 42 cases of lymph node metastasitic tissues. Result:The percentage of p53 positive is 53%in this group of gastric adenocarcinoma patients. The p53 positive rate is significantly higher in poorly differentiated group than in both moderately and well differentiated groups(χ2=7. 486,P<0. 01),significantly higher in these with lymph node metastasis than in these without metastasis(χ2=6. 368,P<0. 05),and significantly higher in these at stage III or VI than in these at stage I or II(χ2=13. 864,P<0. 01). There is no significant difference comparing the positive rates among sub-groups of fundus/cardiac,corpus,antral/pyloric adenocarcinomas. The percentage of NF-kBp65 positive is 58%in the whole group,and there is significant difference between poorly and moderately/well differentiated adenocarcinomas,between these with lymph node metastasis and without metastasis,and between these at stage III or IV and these at stage I or II. The positive rates of p53 and NF-kB are 30. 95%and 35. 71 respectively in 42 cases of patients with lymph node metastasis. These positive rates are significantly lower than in non-metastasis cases(χ2=4. 861,P<0. 05;χ2=8. 052,P<0 . 05 ) . There is a positive correlation between expression of p53 and NF-kBp65 in primary tumor and metastastic lymph node tissues. Conclusion:The abnormal expression of p53 plays a critical role in the development,invision and metastasis of gastric adenocarcinoma. The co-expression of p53 and NF-kB in tumor tissues implies that the interaction of these proteins may be involved in the proliferation, invision and metastasis of gastric adenocarcinoma. Detection of p53 and NF-kBp65 as a pair of biomarkers may provide indicative information for the prediction of tumor development and patient''s prognosis.