37份洋葱遗传多样性的RAPD和SSR分析

Genetic Polymorphism of 37 Onion Germplasm Resources as Revealed by RAPDs and SSRs Markers

利用RAPD和SSR方法研究了37份洋葱种质资源间的遗传多样性,并对这2种分子标记方法进行了比较。结果表明,从15个RAPD引物中,检测到61条有多态性的带;利用筛选出的8对SSR引物,检测到41条多态性带;RAPD和SSR标记均有很高的多态性,RAPD多态性带所占比例为84.7%,SSR位点检测出的平均有效等位基因数为3.75;SSR标记的有效等位基因数、Nei's基因多样性指数及Shannon信息指数的平均值均高于RAPD标记。UPGMA聚类分析表明,2种标记均可将37份洋葱种质资源划分为四大类,聚类结果相似。RAPD和SSR标记均适于洋葱种质的遗传多样性研究,但SSR标记效果较好。

In this paper,the genetic diversity of 37 onions(Allium cepa L.) germplasm resources were analyzed by SSRs and RAPDs markers and the results of these two methods were compared .The results showed that 61 poly-morphic bands were detected by 15 RAPD primers and 41 polymorphic bands were detected by 8 SSR primers .Both RAPDs and SSRs were highly polymorphic .The percentage of polymorphic RAPDs bands was 84 .7%.The average effective number of alleles was 3.75 for SSRs.The average effective number of alleles ,Nei′s genetic diversity index , Shannon information index for SSRs markers were higher than those in RAPDs .UPGMA cluster analysis indicated that both the two markers could divided 37 germplasm into four groups and the classification were fundamentally i-dentical .The study suggested that both RAPDs and SSRs could be used to analyze the genetic diversity of onion germplasm resources ,but the effect for SSRs marker was better than RAPDs .