鸡源蛋白酶产生菌P-98菌株的筛选与鉴定

Screening and Indentification of a Protease Producing Bacteria Strain P-98 from the Broiler

通过初筛从健康青年鸡肠道内容物中分离到67株产蛋白酶菌株,采用酪蛋白平板法进行复筛,并对P-98菌株进行形态特征观察、生理生化试验和16S rDNA基因的序列分析。将该菌株的形态特征和生理生化特性鉴定结果与相应种、属模式菌的性状比对,菌株P-98与芽孢杆菌的相应性状相近。根据16S rDNA基因序列相似性分析,此菌株与Bacillus methylotrophicus标准菌株FZ42相似度达99.78%,因此鉴定该菌株P-98为Bacillus methylotrophicus。

This experiment was conducted to get a strong vitality of the bacterium strain producing protease . Through screening from healthy young chicken intestinal contents were isolated from 67 strains of protease producing strain,and complex sieve used casein plate method .Then the strain P-98 was characterized by morphological and culture features observation ,physiological and biochemical experiments ,and 16S rDNA gene sequence analysis .As the morphology characteristics and physiological and biochemical of the stain were similar to Bacillus and the simi-larity of the 16S rDNA gene sequences between strain P-98 and the type strain was up to 99.78%,the strain P-98 was initially identified as Bacillus methylotrophicus.