无机硫形态及配施对韭菜硝酸盐还原同化的影响

Effects of Combined Application of Different Inorganic Sulfur Forms on Nitrate Reduction and Assimilation in Chinese Chive

以多年生日光温室韭菜为试材,以10 mmol/L的硫为总量,研究了无机硫形态及配施对韭菜硝酸盐含量、氮代谢关键酶活性和营养品质的影响。结果表明:NaHSO3和Na2S2O3配合施用可明显提高硝酸还原酶(NR)、谷氨酸草酰乙酸转氨酶(GOT)、谷氨酸丙酮酸转氨酶(GPT)活性,有效降低硝酸盐含量,其中以7.5 mmol/L NaHSO3+1.25mmol/L Na2S2O3(S6处理)效果最为明显,降低了35.79%。施硫处理后,韭菜叶片可溶性蛋白质、游离氨基酸、可溶性糖、维生素C含量均有不同程度的增加,且以游离氨基酸增加幅度最大,2.5 mmol/L NaHSO3+3.75 mmol/L Na2S2O3(S7处理)和5 mmol/L Na2S2O3(S4处理)分别提高了88.89%和83.33%,并且提高了游离氨基酸与可溶性蛋白比值A/P。同时,NaHSO3和Na2S2O3配合施用也表现出促进韭菜干物质的积累,增加韭菜株高和鲜质量。

With perennial greenhouse Chinese chive as experimental materials ,10 mmol/L sulfur as total,to study the effects of inorganic sulfur forms on the nitrate content of Chinese chive ,nitrogen metabolism and nutrition-al quality.Experimental results show that ,the combined application of NaHSO 3 and Na2 S2 O3 can significantly im-prove the activities of nitrate reductase ( NR) ,glutamic-oxaloacetic transaminase ( GOT) ,glutamic-pyruvic transam-inase ( GPT ) , and reduce the nitrate content effectively , especially , the effect of 7 .5 mmol/L NaHSO 3 +1 .25 mmol/L Na2S2O3 is the best,and it can decrease 35.79%.After sulfur treatment,and the content of soluble pro-tein,free amino acids,soluble sugar,Vitamin C in Chinese chive leaves increases in different degrees ,especially, free amino acids has the greatest increases .2.5 mmol/L NaHSO3 +3.75 mmol/L Na2 S2 O3 ( S7 ) increases 88.89%,and 5 mmol/L Na2 S2 O3 (S4) increases 83.33% and soluble protein (A1P) increase.Meanwhile,the combined application of NaHSO 3 and Na2 S2 O3 can also promote the accumulation of dry matter Chinese chive ,and increase chives plant height and fresh weight .