摘要
目的 观察地塞米松及孟鲁司特对支气管哮喘(哮喘)大鼠模型Eotaxin 及其受体(CCR3)的影响.方法 48只SD大鼠1％卵白蛋白致敏并激发,随机分为4组,哮喘组(A组),地塞米松组(B组),孟鲁司特组(C组),地塞米松+孟鲁司特组(D组).结果 B、C、D组和A组相比,Eotaxin及CCR3蛋白的表达明显下降(P＜0.01);D组和C、B组相比,Eotaxin及CCR3蛋白表达差异有统计学意义(P＜0.05).结论 地塞米松能抑制Eotaxin及CCR3蛋白表达;孟鲁司特能使Eotaxin及CCR3蛋白表达下调;二者合用能使Eotaxin及CCR3蛋白表达明显下降,具有协同作用.
Objective To observe the effects of Dexamethasone and Montelukast on Eotaxin and CCR3 of asthmatic rats.Methods Fourty-eight SD rats were sensitized and challenged by 1％ ovabumin to establish asthmatic model.Results The number of Eotaxin mRNA and Eotaxin protein positive cells as well as the number of CCR3 protein positive cells form group B,C and D were down-regulated significintly,compared to those form group A (P＜0.01).The number of CCR3 protein positive cells in lung tissue and bone marrow form groups B and C,as compared with group D,the difference was significant.Conclusion Dexamethasone probably inhibited the expression of Eotaxin and CCR3; and decreased eosinophil number in the level of bone marrow,Montelukast probably down-regulated them.Montelukast showed apparently synergistic effect with Dexamethasone to inhibit the expression of Eotaxin and CCR3.
出处
《中国临床实用医学》
2014年第1期-,共2页
China Clinical Practical Medicine
