摘要
目的 观察地塞米松及孟鲁司特对支气管哮喘(哮喘)大鼠模型Eotaxin 及其受体(CCR3)的影响.方法 48只SD大鼠1%卵白蛋白致敏并激发,随机分为4组,哮喘组(A组),地塞米松组(B组),孟鲁司特组(C组),地塞米松+孟鲁司特组(D组).结果 B、C、D组和A组相比,Eotaxin及CCR3蛋白的表达明显下降(P<0.01);D组和C、B组相比,Eotaxin及CCR3蛋白表达差异有统计学意义(P<0.05).结论 地塞米松能抑制Eotaxin及CCR3蛋白表达;孟鲁司特能使Eotaxin及CCR3蛋白表达下调;二者合用能使Eotaxin及CCR3蛋白表达明显下降,具有协同作用.
Objective To observe the effects of Dexamethasone and Montelukast on Eotaxin and CCR3 of asthmatic rats.Methods Fourty-eight SD rats were sensitized and challenged by 1% ovabumin to establish asthmatic model.Results The number of Eotaxin mRNA and Eotaxin protein positive cells as well as the number of CCR3 protein positive cells form group B,C and D were down-regulated significintly,compared to those form group A (P<0.01).The number of CCR3 protein positive cells in lung tissue and bone marrow form groups B and C,as compared with group D,the difference was significant.Conclusion Dexamethasone probably inhibited the expression of Eotaxin and CCR3; and decreased eosinophil number in the level of bone marrow,Montelukast probably down-regulated them.Montelukast showed apparently synergistic effect with Dexamethasone to inhibit the expression of Eotaxin and CCR3.
出处
《中国临床实用医学》
2014年第1期-,共2页
China Clinical Practical Medicine