预处理方法对等离子体喷涂氧化钛涂层装载庆大霉素的影响研究

Effects of the treatment methods on the gentamicin loading in plasma sprayed titania coatings

目的本文研究不同预处理方法对等离子体喷涂氧化钛涂层表面庆大霉素的装载量及缓释性能的影响。方法采用大气等离子体喷涂设备在钛合金上制备氧化钛涂层,采用酸碱等化学处理手段预处理涂层,然后在涂层表面嫁接固定庆大霉素,光学显微镜观察胶原在涂层表面的分布情况,采用邻苯二甲醛方法检测释放液中含有的庆大霉素量。结果经1M硫酸或10M氢氧化钠处理后,涂层表面抗菌药物装载量、稳定性皆有明显提高。庆大霉素的胶原溶液比其水溶液更利于其在氧化钛涂层表面的装载。结论酸或者碱处理皆能明显提高涂层表面抗菌药物的装载量及稳定性。这是由于预处理后形成的Ti-OH与胶原形成分子中存在的-COOH、-NH2、-OH等官能团反应,从而将胶原固定在涂层表面所致。

Objective To evaluate the effects of the pre-treatment methods on the gentamicin loading amount and releasing on the plasma sprayed titania coatings. Methods Titania coatings were deposited on titanium alloy substrates by atmospheric plasma spraying. NaOH or H2 SO4 solution was implied to pretreat the surface of the as-sprayed titania. Gentamicin was loaded onto the titania coatings through grafting the collagen with gentamicin. The distribution of collagen on coatings surface was observed under optical microscope, fluorimetric assay with o-phthalaldehyde was used to determine the content of gentamicin in solutions. Results It was found that, apparently higher amount of gentamicin with improved stability was loaded on the titania coatings after treated by 1 M H 2 SO 4 or 10 M NaOH than the as-sprayed one. Collagen solution of gentamicin was beneficial for the loading on the coatings than the water solution. Conclusion The enhanced loading amount of gentamicin was attributed to the formed Ti-OH groups in the coatings after chemical treatments, which can react with the-COOH,-NH 2,-OH groups in the collagen, and thus, a higher stability can be obtained.