豆豉中解淀粉芽孢杆菌发酵产纤溶酶

Production of Fibrinolytic Enzyme by Bacillus Amyloliquefaciens from Douchi

从我国传统食品豆豉中分离出一株高产纤溶酶的细菌XY-1,经生理生化和16S rDNA序列分析,鉴定为解淀粉芽孢杆菌,该菌产生的纤溶酶被命名为豆豉纤溶酶(DFE)。通过P-B设计和响应面法结合应用,有效的优化了发酵培养基成分。当培养基组成为蛋白胨11.4g/L,硫酸镁0.5g/L,NaCl1g/L时,DFE产量的预计最大值为19.78 FU/mL。实际发酵结果表明DFE的最大产量为21.33 FU/mL,为预测值的107.84%,比优化前提高了79.55%。

In this study, we isolated a high fibrinolytic enzyme producing strain XY-1 from Douchi-a traditional Chinese food. According to its physiochemical property and 16S rDNA sequence , it was identified as Bacillus amyloliquefaciens. The fibrinolytic enzyme produced by strain XY-1 was named as Douchi fibrinolytic enzyme (DFE). The culture medium for enzyme producing was optimized by Plackett-Burman and Box-Behnken design. From the response surface model, we predicted the maximal production of 19.78 FU/mL with peptone, magnesium sulfate and sodium chloride at the concentrations of 11.4, 0.5 g/L and 1 g/L, respectively. However, actual maximal yield was about 21.33 FU/mL, equal to 107.84 % of the predicted value, and the yield had increased by 79.55%.