固本宣肺化痰粗提物对阳虚小鼠止咳平喘抗炎作用研究

The Efficacy of Gubengxuanfei Crude Extract on Relieving Cough,Asthma and Anti-inflammation in Yang Deficiency Model Mice

目的研究固本宣肺化痰粗提物(GBXF)对阳虚模型小鼠的止咳、平喘和抗炎作用.方法采用腹腔注射氢化可的松复制阳虚小鼠模型,应用二甲苯致小鼠耳廓肿胀、醋酸致小鼠腹腔毛细血管通透性增加、氨水引咳法、卵白蛋白致喘法,观察固本宣肺化痰粗提物的止咳、平喘、抗炎作用.结果与模型对照组相比,中、高剂量的固本宣肺化痰粗提物可明显减轻二甲苯致小鼠耳廓肿胀度、降低腹腔液的吸光度、明显延长浓氨水所致小鼠咳嗽的潜伏期并减少咳嗽次数,高剂量的固本宣肺化痰粗提物明显延长卵白蛋白诱发小鼠抽搐的潜伏期.结论固本宣肺化痰颗对阳虚小鼠具有明显的抗炎、止咳和平喘作用.

Objective To study the effects of Gubengxuanfei(GBXF) crude extract on relieving cough and asthma and anti-inflammation in yang deficiency model mice(mice lack of vital energy).Methods Yang deficiency model mice were duplicated by intraperitoneal injection of hydrocortisone.The anti-inflammatory effects were investigated with the observation of dimethylxylene-caused mouse ear swelling and the permeability of abdominal capillary in mice respectively.The models of cough and asthma in mice were formed with the use of ammonia water and ovalbumin respectively.Results Compared with model group,the middle and high doses of GBXF significantly relieved the dimethylxylene-caused mouse ear swelling,decreased the permeability of capillary and prolonged the mice coughing latent period frequently induced by stronger ammonia water.High dose of GBXF significantly prolonged spasm latent period induced by ovalbumin.Conclusion Studies indicate that GBXF has a significant effect on relieving cough and asthma and anti-inflammation to yang deficiency model mice.