RASSF1A基因甲基化与胃癌的相关性分析

An analysis of the relationship between the methylation of RASSF1A gene and gastric cancer

目的探讨RASSF1A甲基化与胃癌临床病理资料的关系。方法通过计算机检索PUBMED(1998.01～2012.07)、中国知网(1998.01～2012.07)、万方数据库(1998.01～2012.07)等数据库,收集RASSF1A甲基化与胃癌关系的相关文献,按纳入与排除标准筛选病例对照试验。评价质量及提取资料后,采用RevMan 5.1软件对数据进行Meta分析。结果共检索到36篇,经筛选最终纳入9篇,共506例胃癌组织。Meta分析结果显示,早期(Ⅰ期-Ⅱ期)与中晚期胃癌(Ⅲ期-Ⅳ期)患者胃癌组织中RASSF1A甲基化阳性率合并的OR值为0.36[95%CI(0.22,0.60),P<0.01],中高分化与低分化胃癌组织中RASSF1A甲基化率合并的OR值为0.73[95%CI(0.39,1.34),P>0.05],淋巴结转移与无转移患者胃癌组织中RASSF1A甲基化阳性率合并的OR值为2.07[95%CI(1.19,3.60),P=0.01],男性与女性患者OR值为1.09[95%CI(0.68,1.73),P>0.05],<60岁与≥60岁患者胃癌组织中RASSF1A甲基化阳性率合并的OR值为0.77[95%CI(0.47,1.25),P>0.05],肠型与弥漫型患者胃癌组织中RASSF1A甲基化阳性率合并的OR值为0.95[95%CI(0.37,2.46),P>0.05]。结论胃癌患者病理分期、有无淋巴结转移与胃癌组织RASSF1A甲基化密切相关,中晚期胃癌患者RASSF1A甲基化阳性率高于早期胃癌患者,有淋巴结转移的胃癌患者RASSF1A甲基化阳性率较高。而胃癌患者的性别、年龄、分化程度、Lauren分型与胃癌组织中的RASSF1A甲基化阳性率无显著相关性。

Objective To assess the relationship between the methylation of RASSF1A gene and gender, age,TNMstage,differentiation,lymph node metastasis,Lauren''s type of patients with gastric cancer,and to discuss the significance of RASSF1A gene methylation in gastric cancer.Methods We searched PUBMED(1998.01 ~2012. 07),CNKI(1998.01 ~2012.07),WANFANG data base(1998.01 ~2012.07).Randomized controlled trials(RCTs) in which methylation of RASSF1A gene was detected in the patients with gastric cancer were collected.Then we screened the retrieved studies according to inclusion and exclusion criteria,evaluated the quality of the included stud-ies,and performed meta-analyses by RevMan 5.1 software.Results A total of 36 articles were searched and 9 arti-cles involving 506 patients were finally included.We choosed the fixed-effect model to conduct meta-analysis because heterogeneities were not found among these articles.The different analysis between Ⅰ-Ⅱ stages and Ⅲ-Ⅳ stages showed that the combined OR was 0.36[95%CI(0.22,0.60),P <0.01].The different analysis between well and moderate differentiations and poor differentiation showed that the combined OR was 0.73[95%CI(0.39,1.34),P >0.05].The different analysis between with and without lymph node metastasis showed that the combined OR was 2.07,[95%CI(1.19,3.60),P =0.01].The different analysis between male and female showed that the combined OR was 1.09[95%CI(0.68,1.73),P >0.05].The different analysis between <60 years old and ≥ 60 years old showed that the combined OR was 0.77[95%CI(0.47,1.25),P >0.05].The different analysis between Intestinal type and Diffuse type showed that the combined OR was 0.95,[95%CI(0.37,2.46),P >0.05].Conclusion The TNM stages,differentiation,lymph node metastasis of patients with gastric cancer are closely related with the methyla-tion of RASSF1A.Methylation rate of RASSF1A is higher in Ⅲ-Ⅳstages and in lymph node metastasis,and there is not significant correlation between methylation of RASSF1A and gender,age,differentiation,Lauren''s type.