颈动脉粥样硬化患者MT2A-838G/C和MCP-1-2518G/A基因多态性分析

Analysis of the MT2A-838G/C and the MCP-1-2518G/A gene polymorphism in carotid arteriosclerosis

目的:探讨苏南地区汉族人群MT2A-838G/C和MCP-1-2518G/A基因多态性与颈动脉粥样硬化的相关性。方法:采用碱基淬灭探针技术检测103例有颈动脉斑块患者和46例正常对照的MT2A基因-838位点和MCP-1基因-2518位点基因型分布及基因频率,并评价基因多态性与颈动脉粥样硬化易感性之间的相关性。结果:在对照组和病例组中,MT2A 3种基因型GG、GC、CC的频率分别为52.17%、36.96%、10.87%和50.49%、36.89%、12.62%(P>0.05),等位基因G、C的频率分别为70.65%、29.35%和68.93%、31.07%(P>0.05);MCP-1 3种基因型AA、AG、GC在对照组和病例组中的频率分别为15.22%、52.17%、32.61%和20.39%、44.66%、34.95%(P>0.05),等位基因A、G的频率分别为41.30%、58.70%和42.72%、57.28%(P>0.05)。结论:MT2A-838G/C及MCP-1-2518G/A基因多态性可能不是苏南汉族人群发生颈动脉粥样硬化的独立危险因素。

Objective:To assess the potential association of the MT2A-838G/C and the MCP-1-2518G/A gene in patients with carotid arteriosclerosis(CA) in Chinese han population of Jiangsu.Method:A total of 103 consecutive patients with a diagnosis of CA and compared with 46 healthy controls.The polymorphism were detected by base-quenched probe technique,the genotypes and allele frequencies of MT2A-838G/C and MCP-1-2518G/A were calculated.Results:The genotype GG,GC,CC distribution of MT2A were 50.49%,36.89%,12.62% in patients with CA,and were 52.17%,36.96%,10.87% in controls,respectively(P > 0.05).Allele G and C frequency of MT2A in patients with CA were 70.65%,29.35%,and were 68.93%,31.07% in controls,respectively(P > 0.05).The genotype AA,AG,GC distribution of MCP-1 were 20.39%,44.66%,34.95% in patients with CA,and were 15.22%,52.17%,32.61% in controls,respectively(P > 0.05).Allele A and G frequency of MCP-1 in patients with CA were 41.30%,58.70%,and were 42.72%,57.28% in controls,respectively(P > 0.05).Conclusion:The-838G/C of MT2A gene and the-2518G/A of MCP-1 gene may be not the independent risk factor for CA in Chinese Han population of Jiangsu.