热休克蛋白27、转化生长因子-β_1在甲状腺乳头状癌中的表达及临床意义

Expression of HSP27 and TGF-β_1 in papillary thyroid carcinoma and its significance

目的:探讨热休克蛋白27(heat shock protein 27,HSP27)及转化生长因子β1(transforming growth factor-β1,TGF-β1)在甲状腺乳头状癌中的表达及其临床价值。方法:取外科手术切除甲状腺标本130例,其中甲状腺乳头状癌50例、甲状腺腺瘤50例,正常甲状腺组织30例。样本切片后应用免疫组化技术检测HSP27和TGF-β1的表达。结果:甲状腺癌组HSP27、TGF-β1表达阳性率分别为70.0%(35/50)、80.0%(40/50),甲状腺腺瘤组分别为48.0%(24/50)、62.0%(31/50),正常甲状腺组分别为46.7%(14/30)、56.7%(17/30)。甲状腺癌组HSP27、TGF-β1表达阳性率明显高于甲状腺腺瘤组(P<0.05)及正常甲状腺组(P<0.05)。甲状腺腺瘤组与正常甲状腺组间阳性率无明显差异。结论:HSP27和TGF-β1可能是甲状腺乳头状癌发生、进展及评估预后的重要生物学标志物。

Objective:To investigate the expression and clinic significance of heat shock protein 27(HSP27) and TGF-β1 in papillary thyroid carcinoma.Methods:The expression of HSP27 and TGF-β1 was detected by immunohistochemistry in 50 cases with papillary thyroid carcinoma,50 cases with thyroid adenoma and 30 cases with normal thyroid tissues,which had undergone operation in department of surgery,and confirmed by pathology.Results:The cells which express HSP27 and TGF-β1 were stained brown or dark brown in their cytoplasm.We choose the cells stained biopsy to detect.The positive expression rate of HSP27 was 70%(35/50) with papillary thyroid carcinoma,48%(24/50) with thyroid adenoma,46.7%(14/30) with normal thyroid tissues and of TGF-β1 was 80%(40/50) with papillary thyroid carcinoma,62%(31/50) with thyroid adenoma,56.7%(17/30) with normal thyroid tissues.The positive rates of HSP27,TGF-β1 were significantly higher in the papillary thyroid carcinoma than in thyroid adenoma(P <0.05).The positive rates of HSP27,TGF-β1 were significantly higher in the papillary thyroid carcinoma than in normal thyroid tissues(P < 0.05).The positive rates in the thyroid adenoma was close to the positive rates in normal thyroid tissues(P > 0.05).Conclusion:The expression of HSP27 and TGF-β1 may be important biological markers reflecting the carcinogenesis,progression and prognosis of the papillary thyroid carcinoma.