青钱柳内生真菌培养条件的优化研究

Optimization of culture conditions about endophytic fungi from Cyclocarya paliurus (Batal.) Ijinskaja

对青钱柳内生真菌的培养条件进行优化研究,为进一步研究青钱柳内生真菌代谢产物的化学成分及以此为微生物源的药物开发奠定基础。结果表明,在PDA培养基中添加适量青钱柳茎叶后,从青钱柳茎叶分离到的菌株数量明显增多,青钱柳茎叶最佳添加量为100g/L;液体培养基中添加青钱柳茎叶后,其菌丝体获得量、干菌丝获得率呈增长趋势,青钱柳茎叶的最佳添加量为150g/L。

The optimization culture conditions of endophytic fungi of Cyclocaryapaliurus(Batal.)Ijinskaja is researched, in order to further study the chemical composition of endophytic fungi of Cyclocaryapaliurus(Batal.)Ijinskaja metabolites and to lay the foundation for microbial source of drug development.Results show that strains isolated from Cyclocaryapaliurus(Batal.)Ijinskaja stem leaf increased obviously by adding stem leaf in PDA,while stem leaf best add quantity is 100g/L.The mycelium gain rate,dry mycelium are rise when liquid medium adding Cyclocaryapaliurus(Batal.)Ijinskaja stem leaf,and the best addition amount of 150g/L.