茯苓止痒丸的质量标准研究

Study on Quality Standard of Poria Antipruritic Pellet

目的：探讨茯苓止痒丸的质量控制方法。方法：采用薄层色谱法（TLC）定性鉴别茯苓止痒丸中的苍术、黄柏及甘草，用高效液相色谱法（HPLC）测定茯苓止痒丸中的盐酸小檗碱含量。结果：苍术、黄柏、甘草具有鉴别特征，阴性无干扰；盐酸小檗碱在0.4152~2.076mg范围内呈良好的线性关系，r=0.9999，平均加样回收率为99.37%，RSD=0.36%。结论：薄层色谱法及高效液相色谱法简便准确，重现性好，可作为茯苓止痒丸的质量控制标准。

Objective:To explore the quality control method of poria antipruritic pellet. Methods:Rhizoma atractylodis, golden cypress and liquorice of poria antipruritic pellet were qualitatively identified with thin layer chromatography(TLC), and the content of berberine hydrochloride in poria an-tipruritic pellet was measured with high performance liquid chromatography(HPLC). Results: Rhizoma atractylodis, golden cypress and liquorice with distinguishing characteristics was negative without interference; berberine hydrochloride has a good linear relation in the range between 0.4152mg and 2.076mg, r=0.9999, the average rate of sample recovery was 99.37%, RSD=0.36%. Conclusion:TLC and HPLC are simple, accurate and reproducible, which can be used as the quality control standard for poria antipruritic pellet.