生物活性玻璃对早期人工釉质龋的再矿化

Bioactive glass effects on remineralization of early artificial enamel caries

背景:生物活性玻璃具有良好的生物相容性,且具有抑制口腔致龋细菌和牙周相关细菌及抗牙本质过敏的作用。目的:评价生物活性玻璃促进早期人工釉质龋再矿化的作用。方法:将新鲜拔除的30颗牛切牙制成人工龋模型,将标本在37℃人工脱矿液内脱矿72h,用扫描电镜观察脱矿后釉质表面的平滑情况,用显微硬度仪测量脱矿后釉质的显微硬度。然后随机分为3组,每组10个。采用pH循环法模拟人口腔环境,将3组标本分别浸泡在生物活性玻璃溶液、氟化钠溶液及人工唾液内,3次/d,10min/次,循环浸泡20d,扫描电镜检测标本脱矿及再矿化情况,用显微硬度计检查牙釉质显微硬度。结果与结论:浸泡在生物活性玻璃溶液中的牙釉质表面较浸泡在其他两溶液中的牙釉质表面光滑平整,无空隙存在;浸泡在生物活性玻璃溶液中的牙釉质表面显微硬度高于浸泡在其他两溶液中的牙釉质表面显微硬度(P<0.05)。说明生物活性玻璃在体外实验中能促进早期釉质龋的再矿化。

BACKGROUND: Bioactive glass has good biocompatibility and could control oral bacteria and protect against dentin hypersensitivity. OBJECTIVE: To evaluate remineralization effect of bioactive glass on early artificial enamel caries. METHODS: Thirty bovine incisors fresh were made to artificial caries models, and were placed in a container with demineralization iquid at 37 ℃ for 72 hours. Micro hardness tester was used to detect hardness value after demineralization, and the enamel surfaces were observed by scanning electron microscope. Then, the samples were randomly divided into three groups, 10 in each group. Using pH cycle way method, the oral environment of human beings was copied. Samples were respectively soaked in bioactive glass solution, sodium fluoride solution and artificial saliva solution, three times a day and 10 minutes each. These three groups were dipped circularly into an artificial cariogenic solution and an artificial saliva solution for 20 days. The scanning electron microscope was used to observe demineralization and remineralization on the enamel surface. Micro hardness tester was employed to detect enamel hardness. RESULTS AND CONCLUSION: The enamel surface in the experiment group was smoother than the other two control groups. The micro hardness of the experiment group was higher than that of the two control groups (P < 0.05). Therefore, bioactive glass can promote remineralization of early artificial enamel caries in vitro.