CDK8和MMP-7在结直肠癌组织中的表达及相关性分析

Expressions and correlation analysis of CDK8 and MMP-7 in colorectal cancer tissues

目的检测结直肠癌患者中CDK8与MMP-7蛋白及mRNA的表达,分析其相关性及其表达与患者临床病理特征的关系。方法使用免疫组织化学法及RT-PCR法检测肿瘤组织和癌旁组织及正常组织中CDK8、MMP-7蛋白及mRNA的表达情况,分析其差异性表达,同时分析肿瘤组织中CDK8与MMP-7表达的相关性及其与临床病理特征的关系。结果结直肠癌患者肿瘤组织中CDK8、MMP-7蛋白及mRNA表达水平均高于癌旁组织及正常组织(P<0.05),肿瘤组织中CDK8蛋白及mRNA表达水平与MMP-7表达呈正相关(P<0.05)。CDK8蛋白表达与肿瘤浸润程度、Duke分期、有无淋巴结及远处转移显著相关(P<0.05),与患者年龄、性别、肿瘤位置、分化程度无显著相关(P>0.05)。MMP-7表达量与肿瘤浸润程度、Duke分期、有无淋巴结转移相关(P<0.05),与患者年龄、性别、肿瘤位置、分化程度及远处转移无显著相关(P>0.05)。结论 CDK8是癌基因,可上调MMP-7蛋白表达,促进肿瘤浸润、侵袭转移,高表达提示患者预后不良。

Objective To detect the expressions of cyclin-dependent proteinkinase 8(CDK8) and matrix metalloproteinases 7(MMP-7) in colorectal cancer tissues, and investigate their correlation and relationship with clinicopathologic features of colorectal cancer patients. Methods Immunohistochemical(IHC) method and RT-PCR were used to detect CDK8 and MMP-7 protein and mRNA expressions in tumor tissues,tissues adjacent to cancer from colorectal cancer patients and normal tissues..The correlation between CDK8 and MMP-7 expressions and relationship of CDK8 and MMP-7 expressions with clinicopathologic characteristics were analysized. Results Both protein and mRNA expressions of CDK8 and MMP-7 in tumor tissues were significantly higher than those in tissues adjacent to cancer and normal tissues(P <0.05)..Both protein and mRNA expressions of CDK8 were positively correlated with MM P-7expressions respectively(P <0.05). CDK8 Expression was significantly correlated with tumor infiltrating degree, Duke's stages, lymph node and distant metastasis(P<0.05), but not with age, sex, tumor location and differentiation(P>0.05). MMP-7 expression was significantly correlated with tumor infiltrating degree,Duke's stages and lymph node metastasis(P<0.05),but not with age,sex,tumor location,tumor differentiation and distant metastasis(P>0.05). Conclusions CDK8 is an oncogene, which can up-regulate MMP-7expression and promote tumor infiltration, invading and metastasis. High expressions of CDK8 and MMP-7indicate that the colorectal cancer patients have a poor prognosis.