出境介质盆景线虫分离检验方法的改进试验

Improve Experiment on Nematode Isolation Method for Export Bonsai Medium

介质盆景线虫的分离检验是出口盆景检疫的一项重要工作 ,试验以改进浮力离心法〔1〕为基础 ,采用浸泡浮力离心法对介质线虫进行分离检验 ,比较此法各种浸泡时间、漏斗法、浅盆法检验线虫的回收量 .结果表明 ,浸泡 16h ,6h的浮力离心法的线虫回收效果比漏斗法、浅盆法分别高 15 5 67% ,43 68%和 96 7% ,10 5 5 % ,该方法综合了浅盆法 (漏斗法 )和改进浮力离心法的优点 ,既促使线虫游离于介质中 ,分离出较多线虫 ,提高介质线虫检出率 ,同时也缩短了线虫检验结果时间 ,该方法已被推广应用于出口盆景介质的线虫检验 ,收到良好效果 .

Inspection of nematode isolation for bonsai medium is an important work in export bonsai quarantine, this experiment basic on the improve float centrifuge method(Guo Quan et al, 1995), use the Soak float centrifuge method to isolate nematodes inside the medium, compare nematode recovery amount between soak time of this method and Funnel method or Pans method. The result showed that Soak float centrifuge method prior to soak the medium for 6,16 hours could recover nematodes 155 67%,43 68% and 96 7%,10 55% higher than Funnel method and Pans method respectively. the method combine the good point of Pans method (Funnel method) and the improve float centrifuge method, it not only spur nematodes separate to medium, isolate more nematode, increase medium nematode recovery rate, but also shorten time for inspection nematode. The method has been applied to work on nematode inspection for export bonsai medium, achieve a good result.