筛选根际促生细菌防治蔬菜根结线虫病

Screening Rhizobacteria to Control Root knot Nematodes and to Promote Growth of Vegetable Crops

自武汉市蔬菜基地 30种蔬菜作物的根际土壤中共分离了细菌分离物 1334株 .室内检测其对目标根结线虫 (Meloidogyneincognita) 2龄幼虫的致死作用 ,获得 32株致死率 80 %以上的有效菌株 ,占总细菌分离物的 2 4% .盆栽促生试验表明 :此 32株有效菌株中有 9株对番茄的生长表现出了明显的促进作用 .以菌悬液 ( 10 9CFU mL)浸根对此 9株菌株进行进一步促生防虫试验 ,得到 4株兼具促生防虫效果的根际细菌分离物 ,其防效均在 60 %以上 .

isolates of rhizobacteria were obtained from rhizosphere of 30 vegetable crops in Wuhan,Hubei and their antaganistic abilities to Meloidogyne incognita 2nd stage juvenile were tested. Among them, only 2 4% of the isolates, namely, 32 strains caused more than 80% mortality to nematodes. In the next experiment, it was found that 9 from 32 strains promoted tomato growth when it was treated by them. In the greenhouse experiment, the abilities to promote tomato growth and to control root knot disease of all the 9 strains were tested. The results showed that 4 out of 9 strains had the double functions .The four strains promoted tomato growth significantly and biocontrolled root knot disease caused by M incognita up to more than 60%.