摘要
13.1 Leukocyte and leukocyte function2007252 Expression of AML1/ETO9a isoform in acute myeloid leukemia-M2 subtype. MIAO Yuqing(苗雨青),et al. The 1st Affili Hosp, Soochow Univ, Jiangsu Hematol Instit, Suzhou 215006. Chin J Hematol 2007;28(1):27-29. Objective To investigate the expression of AML1/ETO9a isoform in the acute myeloid leukemia (AML)-M2 patients. Methods Expressions of AML1/ETO fusion gene and AML1/ETO9a isoform were detected by using reverse transcriptase-polymerase chain reaction (RT-PCR) in leukemia patients, MDS patients, leukemia cell lines and healthy subjects. Karyotype was studied by R-banding technique. Result In 30 newly diagnosed AML-M2 patients, 15 were found to express AML1/ETO9a isoform, while the rest including 20 AML-M2CR, 18 other subtypes of AML, 5 chronic myelogenous leukemia(CML), 3 myelodys-plastic syndromes (MDS), 3 leukemia cell lines (NB4, KG-1, K562) and 5 healthy subjects were AML1/ETO9a negative. Among the 15 AML/ETO9a isoform expressing cases, 13 were demonstrated t(8;21) translocation and AML1/ETO expression. Conclusion lsoform AML1/ETO9a related with AML/M2, may promote the development of leukemia in combination with the AML1/ETO fusion gene.
