摘要
9.1 Heart function and hemodynamics 2006336 Effect of cyclosporine on rat myocardial function. ZHI Ji-xin, et al. Dep Cardiol, The 4th Hosp, Harbiin Med Unive, Harbin 150001. Chin J Endemiol 2006;25(3):260-263. Objective:To evaluate the effect of cyclosporine A(CsA) on the normal rat myocardium heart function and calcineurin (CAN) activity and CaNAβ mRNA expression. Methods:Seventy wistar rats were divided into A,B,C,D and control groups according to the doses of CsA. 2 and 4 weeks after CsA 5.0,12.5,25.0,50.0 mg·kg -1·d -1 was gastrically instilled into A,B,C and D groups for 2 and 4 weeks, the pathological changes were observed. Colorimetric assay was applied for determining CaN enzyme activity. The information of rat left ventricle (LV) and left atrium (LA) value obtained from echocardiography was recorded and CaNAl3 mRNA products by RT-PCR were detected. Results:CsA suppressed heart function by observing both echocardiographic changes and histopathological myocardial degeneration, left ventricle ejection fraction (LVEF,P<0.01) and fraction short(FS, P<0.01) were detected, the enlargement of LV of A,B or C groups and LA of B,C groups (P<0.05) were observed. There was a significant surpress for CaN activity during the 2-week treatment with CsA (F=27.19,P< 0.05), but it was increased after 4 weeks (P<0.05). The expression of CaNAβ mRNA was decreased along with the increase of dose and time (P<0.01).
9.1 Heart function and hemodynamics 2006336 Effect of cyclosporine on rat myocardial function. ZHI Ji-xin, et al. Dep Cardiol, The 4th Hosp, Harbiin Med Unive, Harbin 150001. Chin J Endemiol 2006;25(3):260-263. Objective:To evaluate the effect of cyclosporine A(CsA) on the normal rat myocardium heart function and calcineurin (CAN) activity and CaNAβ mRNA expression. Methods:Seventy wistar rats were divided into A,B,C,D and control groups according to the doses of CsA. 2 and 4 weeks after CsA 5.0,12.5,25.0,50.0 mg·kg -1·d -1 was gastrically instilled into A,B,C and D groups for 2 and 4 weeks, the pathological changes were observed. Colorimetric assay was applied for determining CaN enzyme activity. The information of rat left ventricle (LV) and left atrium (LA) value obtained from echocardiography was recorded and CaNAl3 mRNA products by RT-PCR were detected. Results:CsA suppressed heart function by observing both echocardiographic changes and histopathological myocardial degeneration, left ventricle ejection fraction (LVEF,P<0.01) and fraction short(FS, P<0.01) were detected, the enlargement of LV of A,B or C groups and LA of B,C groups (P<0.05) were observed. There was a significant surpress for CaN activity during the 2-week treatment with CsA (F=27.19,P< 0.05), but it was increased after 4 weeks (P<0.05). The expression of CaNAβ mRNA was decreased along with the increase of dose and time (P<0.01).
