摘要
15.1 Erythrocyte and erythrocyte function 2006398 Effects of interferon on the expressions of TRAIL and other apoptosis-inducing genes in peripheral blood mononuclear cells from polycythemia rubra vera. LIU Yan-fang, et al. Dept Hematol, The 1st Teaching Hosp, Zhengzhou Univ, Zhengzhou 450052. Chin J Hematol 2006;27(3):323-326. Objective:To investigate the effects of interferon (IFN) on the natural killer (NK) cytotoxicity and the expression of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and other apoptosis-inducing genes in peripheral blood mononuclear cells (PBMNC) from polycythemia rubra vera (PV). Methods:PBMNC were collected from 12 PV patients. The NK cytotoxicity was assessed by lactate dehydrogenase release assay and lytic units (LU) were calculated based on specific lysis. The TRAIL mRNA and other apoptosis-inducing genes were determined by RNase protection assay. Results:The NK cytotoxicity of untreated PBMNC from PV was (152.0±146.6) LU. And of IFNα1b- and IFNα2b-treated PBMNC were up to (250.9±197.4 ) LU and (355.9±249.9) LU, respectively (P<0.05 and P<0.01, respectively). The expression of TRAIL mRNA was upregulated in IFNα1b and IFNα2b stimulated-PBMNC. Up-regulation of mRNA levels of FLICE, DR3, DR4 and TNFRp55 genes was observed in PBMNC after stimulated with IFNα, which also induced the mRNA expressions of FasL, Fas, TRADD and RIP.
15.1 Erythrocyte and erythrocyte function 2006398 Effects of interferon on the expressions of TRAIL and other apoptosis-inducing genes in peripheral blood mononuclear cells from polycythemia rubra vera. LIU Yan-fang, et al. Dept Hematol, The 1st Teaching Hosp, Zhengzhou Univ, Zhengzhou 450052. Chin J Hematol 2006;27(3):323-326. Objective:To investigate the effects of interferon (IFN) on the natural killer (NK) cytotoxicity and the expression of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and other apoptosis-inducing genes in peripheral blood mononuclear cells (PBMNC) from polycythemia rubra vera (PV). Methods:PBMNC were collected from 12 PV patients. The NK cytotoxicity was assessed by lactate dehydrogenase release assay and lytic units (LU) were calculated based on specific lysis. The TRAIL mRNA and other apoptosis-inducing genes were determined by RNase protection assay. Results:The NK cytotoxicity of untreated PBMNC from PV was (152.0±146.6) LU. And of IFNα1b- and IFNα2b-treated PBMNC were up to (250.9±197.4 ) LU and (355.9±249.9) LU, respectively (P<0.05 and P<0.01, respectively). The expression of TRAIL mRNA was upregulated in IFNα1b and IFNα2b stimulated-PBMNC. Up-regulation of mRNA levels of FLICE, DR3, DR4 and TNFRp55 genes was observed in PBMNC after stimulated with IFNα, which also induced the mRNA expressions of FasL, Fas, TRADD and RIP.
