联用维甲酸及氟脲嘧啶脱氧核苷治疗肝癌的实验研究

The study of all trans-retinoic acid combined with floxuridine in the treatment of hepatic cell line SMMC7721

【目的】比较全反式维甲酸 (ATRA)和氟脲嘧啶脱氧核苷 (FUDR)单独作用或联合作用肝癌细胞的效应 ,为临床治疗肝癌的用药方案提供理论依据。【方法】以ATRA和FUDR分别及联合作用于培养的肝癌SMMC772 1细胞 ,以Hoechst33342 /PI双荧光活染技术、琼脂糖凝胶电泳 ,流式细胞光度术 (FCM )检测细胞凋亡。【结果】单独作用时 ,ATRA诱导的凋亡细胞数比FUDR组少 ,联用FUDR和ATRA时 ,引起的凋亡细胞数增加 ,染成红色的细胞数也增加 ,FCM检测结果与染色观察结果相符。【结论】多种方法检测结果表明 ,A TRA和FudR对肝癌SMMC772 1细胞的作用以诱导细胞凋亡的形式为主 ,可能是其抗癌的重要机制之一 ,且联合用药比单独用药的药效强 ,具协同增效作用 。

ObjectiveIn order to investigate the influence of all trans-retinoic acid (ATRA) and floxuridine (FUDR) on hepatic cell line SMMC7721.ATRA and FUDR had a single or co-effection on SMMC7721 cell line.The apoptosis of SMMC7721 was detected by fluorescence microscope,electrophoresis and flow cytometry (FCM).ATRA and FUDR could induce the apoptosis of hepatic cell line SMMC7721 respectively. The apoptotic number of co-effection was much more than those of single effection.[Conclusion]The influence of ATRA and FUDR on SMMC7721 was inducing apoptosis .The co-effection was more intense than single effection.ATRA was in coordination with FUDR.A new method in the therapy of liver carcinoma was supplied by this study.