QuEChERS-UPLC-MS/MS快速测定生鲜牛乳中的氟喹诺酮类药物残留

Rapid Determination of Fluoroquinolones Residues in Raw Bovine Milk by UPLC-MS/MS via QuEChERS Pretreatment Method

本试验建立了生鲜牛乳中8种氟喹诺酮类药物的超高效液相色谱串联质谱的测定方法。生鲜牛乳经QuEChERS萃取剂提取,QuEChERS净化剂净化后,经氮气流吹干浓缩后,以超高效液相色谱串联质谱测定,稳定同位素内标法定量。本方法对氟喹诺酮类药物的测定线性范围为2.0~100μg/kg。在2.0、10、100μg/kg低、中、高3个浓度的回收率为80%~120%。批内、批间精密度小于20%。本方法简便、快速、灵敏,适用于生鲜牛乳中氟喹诺酮类药物残留的大批量筛选和定量测定。

An UPLC-MS/MS method was developed and established for rapid determination of fluoroquinolones residues in raw bovine milk.The sample was extracted by QuEChERS extraction Kit,and cleaned up by QuEChERS clean-up tube.An aliquot sample of 5mL was concentrated by nitrogen gas.The analytes were determined by UPLC-MS/MS and quantified by stable isotope internal standard.The results showed good linearity in the ranges of 2.0to 100μg/kg for all fluoroquinolones.The method recoveries were between 80%and 120%,and also the precisions were lower than 20%.The method is simple,rapid,and sensitive.It is suitable for screening of fluoroquinolones residues in raw bovine milk and quantitative determination.