摘要
将抗原、抗体生物芯片用于生鲜乳药物残留的检测中目前相关报道较少,现已商品化的芯片主要有各类抗生素芯片组合、驱虫剂芯片组合、生长促进剂组合等。本试验选用其中能同时定量测定15种磺胺组合的芯片进行研究,考察在生鲜乳药物残留检测中该芯片的准确性、精密度等是否能满足检测的要求。结果发现,本方法线性范围为0~20ng/mL,2个水平的加标(2倍检出限与5或10倍检出限添加量)回收试验其回收率大都在80%~120%之间,其中加入后者回收率优于前者;通过对前一水平加标10次进行测定,结果发现其变异系数大都在5%~15%之间。通过对阴性样品测定全部低于相应的检出限,质控样品测定结果为参考值的80%~110%。表明磺胺生物芯片各项性能指标完全能满足生鲜乳药物残留半定量的快速筛查,为批量样品筛查提供了可靠的技术保证。
The antigen,antibody microarray for detection of drug residues in raw milk were currently very few relevant reports,were now commercially available chip portfolio that there were all kinds of antibiotics,insect repellent combination chip,growth-promoting agent and combination.In this experiment,which could simultaneously quantitative determination of 15kinds of combinations of sulfonamides chip experimental study,drug residues in raw milk in the chip accuracy,precision and so on would be studied.The results showed that the linear range was 0to 20ng/mL,two horizontal spiked(2times the detection limit and 5or 10times the detection limit dosage),their recoveries mostly were in the 80%to 120%,which the latter was better than the former,through previous levels spiked 10times measurement results,the coefficient of variation mostly was in the 5%to 15%.Through the determination of all the negative samples below the respective detection limits,quality control samples were measured to the reference value of 80%to 110%.Prove sulfa biochip could meet the performance indicators of drug residues raw milk semi-quantitative rapid screening,screening for bulk samples to provide a reliable technical guarantee.
出处
《中国畜牧兽医》
CAS
北大核心
2013年第S1期57-61,共5页
China Animal Husbandry & Veterinary Medicine