超高效液相色谱法测定原料乳中羟脯氨酸

Determination of Hydroxyproline in Raw Milk by Ultra High Performance Liquid Chromatography

本试验旨在研究利用超高效液相色谱测定原料乳中羟脯氨酸(Hyp)含量的方法。在原料乳中羟脯氨酸测定过程中各种条件对测定结果的影响,并获得了较好的测定条件。结果发现本方法的回收率在94.7%~98.6%之间,在1.0~16.0μg/mL标准曲线的回归方程为y=3286.5x+87.943,R2=0.9999,相对标准偏差(RSD)小于2.36%;最低检出限为1mg/kg。该方法操作简便、色谱分离速度快、准确度高、精密度好,可用于原料乳中羟脯氨酸的定量测定,从而判定是否在原料乳中添加动物水解蛋白。

This paper was aimed at using ultra high performance liquid chromatographic to determine hydroxyproline(Hyp)content in raw milk.Determination of Hyp in various conditions in raw milk on the effect of determination results obtained better conditions.The recovery rates of this method were 94.7%to 98.6%,1to 16μg/mL regression equation of standard curve was y=3286.5x+87.943,the correlation coefficient was r=0.9999,RSD less than 2.36%.The minimum detection limit was 1.0mg/kg.The method had the simple operation,chromatographic separation speed,high accuracy,good precision,and could be used for quantitative determination of Hyp in raw milk,so as to determine whether hydrolyzed animal protein was added in raw milk.