响应面法优化提取柳树叶化感物质及抑藻效应

Optimization of extraction and identification of allelochemical from leaves of Salix babylonica by response surface methodology

以柳树叶为原料,采用超声提取的方法提取其抑藻化感物质,并通过响应面法对乙醇体积分数、超声提取时间、料液比和温度等条件优化,并通过GC/MS鉴定其主要抑藻物质为对羟基肉桂酸乙酯等酚酸类物质。得到提取过程最佳实验条件:乙醇体积分数为72.92%,时间为160 min,液料比为40.03:1 mL/g,超声温度为55℃。在此最佳条件下,柳树叶化感物质对蛋白核小球藻的抑制率为91.34%,与模型拟合的响应值93.52%较相近,拟合得到的模型较好地符合实际;柳树叶质量浓度为0.1~0.4 g/L时对蛋白核小球藻的抑制效果随着化感物质体积分数的增加而增大。

Ultrasound was used to investigate the effects of ethanol concentration,extraction time,ratio of liquid to solid,and temperature on allelochemical extraction from the leaves of Salix babylonica.The extraction parameters were optimized through the response surface method(RSM).The isolated fractions were characterized by GC/MS,and phenolic acids were identified as the most potential allelochemicals,especially Hydroxy ethyl cinnamate.The results show that the optimized conditions are as follows: The volume fraction of ethanol concentration is 72.92%,the extraction time is 160 min,the ratio of liquid to solid is 40.03:1 mL/g,and the extraction temperature is 55 ℃.Under the optimized inhibition,the rate can reach 91.34% in the verification experiment,which is close to the response value(93.52%) with the model fitting.The optimized model by RSM agrees well with the practice.The inhibition of Chlorella pyrenoidosa increases with the increase of phenolic acids mass concentration at 0.1 0.4 g/L.