摘要
目的:建立一种测定中药保健品中非法添加激素类西药成分的检测方法.方法:采用高效液相色谱法检测中药保健品中非法添加的激素类西药成分。色语柱为C18柱,以乙腈一水为流动相进行梯度洗脱,检测波长为240 nm,流速为1.0 mL/min,柱温为室温,进样量10μL。结果:在整个线性范围之内,十种激素所包括的成分浓度与其峰面积形成较好的线性关系,相关系数均为0.9999,最小检出量范围为20-32 ng/mL。结论:本法操作简便,测定结果准确可靠,可快速筛查出纯中药制剂或食用保健品中的激素类西药成分。
Objective:To establish a method for the simultaneous determination of ten constituents of hormone analogues in traditional Chinese medicine.Methods:The chromatographic column of C18 was used.The mobile phase consisted of acetonitrile-water and the flow rate was 1.0 mL/min.The detection wavelength was at 240 run.Results The correlation coefficient was 0.999'9.The minimal detection amount ranged 20-32 ng/mL.Conclusion:This method is simple,quick and the result of determination is accurate.This method is suitable for the determination of the ten constituents of hormone anologues in traditional Chinese medicine.
出处
《求医问药(下半月)》
2013年第7期163-164,共2页
Seek Medical and Ask The Medicine
关键词
高效液相色谱法
保健品
激素
Traditional Chinese medicine
Health care product
hormone