l5-脱氧-△^(12,14)-前列腺素J2对兔耳增生性瘢痕转化生长因子-β1及微血管的影响

Effect of peroxisome proliferator-activated receptor-γactivator 15d-PGJ2 on hypertrophic scar in rabbit ear

目的观察过氧化物酶体增殖物激活受体-γ的配体l5-脱氧-△12,14-前列腺素J2对兔耳增生性瘢痕CD34及转化生长因子-β1(transforming growth factor-β1,TGF-β1)的影响,探讨15d-PGJ2防治增生性瘢痕的机制和可行性。方法选取新西兰大白兔9只,在兔耳腹侧面制作2cm×2cm全层皮肤缺损创面,每耳2个,共计36个,建立兔耳增生性瘢痕动物模型,随机分为实验组和对照组,分别用15d-PGJ2及生理盐水行瘢痕内注射,1次/d,连续注射7d。停止注射后第3、6、17天取材。应用免疫组织化学方法检测CD34和TGF-β1的表达。结果兔耳创面愈合后均出现不同程度类似人增生性瘢痕组织块,与对照组比较,实验组注射15d-PGJ2后瘢痕逐渐变平变软,颜色变浅。在各时间点CD34及TGF-β1的表达均较对照组低,差异有统计学意义(P<0.05)。结论 PPAR-γ的配体15d-PGJ2可减少瘢痕内CD34、TGF-β1的含量,引起瘢痕萎缩,有一定的防治瘢痕增生的作用,为其治疗增生性瘢痕的临床应用提供了一定的理论依据。

Objective To investigate the effect of 15d-PGJ2on the expression of CD34,TGF-β1andα-SMA on hypertrophic scar in rabbit ear and the possibility of hypertrophic scar treated by 15d-PGJ2.Methods 9New Zealand white rabbits were used to establish a hypertrophic scar model on the rabbit ear.The wounds were established as follows:2cm×2cm wounds with total skin loses on the ventral side,2wounds for each ear,totally 36 wounds.The wounds were randomly divided into the 15d-PGJ2treatment group and normal saline control group.10μL 15d-PGJ2or normal saline was injected into the ear scar once a day for 7days.At 3,6and 17days after the injection.The expression of CD34and TGF-β1was detected by immunohistochemical method.Results Excessive dermal scars on rabbit ear that were similar to human hypertrophic scar appeared in the two groups.Compared with the NS-treated scar group,the 15d-PGJ2-treated scars appeared to be smaller,softer,flatter and lighter in color.The expression of CD34and TGF-β1in the 15d-PGJ2group was significantly decreased as compared with that in the control group at different time points(P<0.05).Conclusion 15dPGJ2,the ligand of PPAR-γ,can reduce the expression of CD34and TGF-β1of hypertrophic scar in the rabbit ear and plays an important role in the prevention and treatment of hypertrophic scar.