响应曲面法优化羊肚菌富硒深层发酵条件

Response surface methodology optimization of Morchella mycelium selenium enriched fermentation

以亚硒酸钠为无机硒源,对羊肚菌菌丝体的富硒深层发酵条件进行了优化研究。选择培养温度、发酵时间和硒浓度作为优化因素,研究各因素的不同水平对富硒羊肚菌菌丝生物量和硒含量的影响。通过单因素试验和响应曲面法得出了羊肚菌菌丝体富硒深层发酵的最佳条件条件:发酵时间5.22d、培养温度25.73℃、硒质量浓度86.67μg/ml;预测菌丝生物量的达到10.512g/L、硒含量达到18.8μg/ml,实际菌丝生物量的达到10.339g/L、硒含量达到18.6μg/ml。研究结果表明,响应曲面法可对羊肚菌富硒深层发酵条件进行优化。

Sodium selenite was used as inorganic selenium source in Morchella mycelium enriched selenium submerged fermentation.The optimal fermentation parameters were researched.Selected culture temperature,fermentation time and selenium concentration as the optimized factors.Influence of levels of the factors on enriched selenium Morchella mycelium biomass and the selenium content were studied.Through the single factor tests and response surface method the optimal processing conditions of Morchella mycelium enriched selenium submerged fermentation was concluded.They are fermentation time 5.22d,culture temperature 25.73 ℃,selenium concentration 86.67 μg/ml;Prediction of mycelium biomass is 10.512 g/L,selenium content 18.8 μg/ml.Actual mycelium biomass is up to 10.339 g/L,selenium content 18.6 μg/ml.The results show that the response surface method is a most useful tool in Morchella selenium-rich submerged fermentation conditions optimization.