胃癌组织中Nodal蛋白表达及意义研究

Expression and significance of Nodal protein in human gastric carcinoma

目的检测Nodal蛋白在正常胃黏膜、胃癌前病变及胃癌中的表达情况,并探讨其在胃癌发生和转移过程中的作用。方法采用免疫组化二步法检测135例人胃癌组织、配对的99例正常胃黏组织、18例慢性萎缩性胃炎组织、34例肠上皮化生组织以及4例异型增生组织中Nodal蛋白的表达情况。结果胃癌组织中Nodal蛋白阳性表达率(69.6%)显著高于正常黏膜(22.2%)和慢性萎缩性胃炎(33.3%)(P<0.05)。肠上皮化生中(58.8%)Nodal阳性表达率也显著高于正常胃黏膜(P<0.05)。Nodal蛋白在有远处转移胃癌(80.4%)中阳性表达率显著高于无远处转移(62.0%),TNM分期Ⅳ期(80.4%)显著高于Ⅲ期(54.8%)(P<0.05)。结论 Nodal蛋白可能作为致癌因子参与了胃黏膜上皮细胞的恶性转化过程,可能参与了胃癌的侵袭和转移。

Objective To detect the expression of Nodal in normal gastric mucosa,chronic atrophic gastritis(CAG),intestinal metaplasia(IM),dysplasia(Dys),gastric carcinoma(GC),and to analyse the differences of their expressions,and to explore the relations to gastric carcinogensis and metastasis.Methods PV9000 immunohistochemical method was used to detect the expression of Nodal protein in 135 gastric carcinomas,99 normal gastric mucosa,18 chronic atrophic gastritis,34 intestinal metaplasia and 4 dysplasia.Results The positive rate of Nodal expression was significantly higher in gastric carcinoma(GC)(69.6%) than in normal gastric(22.2%),chronic atrophic gastritis(CAG)(33.3%),and in intestinal metaplasia(58.5%) higher than normal gastric mucosa,P < 0.05.In TNM staging of gastric cancer,the positive rate of Nodal in gastric cancer of TNM Ⅳ(80.0%) was significantly higher than that in TNM Ⅲ(62.0%),P < 0.05.Conclusion Our results indicated that Nodal may participate in the development,invasion and metastasis of gastric carcinoma.