活性霉菌制剂生产过程中雅致放射毛霉发酵培养基的优化

Optimization of aetinomueor elegans fermentation medium on production of mould starter

采用响应面分析法对雅致放射毛霉发酵培养基进行了优化。采用Plackett-Burman实验确定了麦芽糖、牛肉膏与初始pH这三个因素对菌体的生物量影响显著。采用Box-Behnken设计和响应面优化得到最佳发酵培养基,生物量从未优化前的7.19g/L升高到了8.22g/L。

The formation materials of Actinomucor elegans were optimized by the response surface methodology. First,Plackett-Burman design was adopted to screen out the important factors affecting the biomass they are maltose,beef extract,pH. At last the optimized medium was obtained by Box-Behnken design and response surface methodology.Under the condition,the biomass was increased to 8. 22g / L compared with the previous condition of 7. 19g / L.