一株高生物量链带藻分离筛选与培养条件优化

Optimization of Environmental Factors for Biomass Production of Isolated Strain of Desmodesmus sp.EJ12-3

为了得到具有高生物量累积特性的微藻及其最优培养条件,从自然水样中分离筛选出一株生物量较高的微藻属,经18s rRNA及ITS1鉴定为链带藻属(Desmodesmus sp.),命名为EJ12-3。通过单因素试验及响应面分析对其培养条件进行优化,得到最大生物量累积的条件为:温度28℃,光照强度131μmol/(m2·s),光周期15∶9(光暗时间比),pH值为6,在此条件下,其培养14 d后的生物量累积可达(0.648±0.015)g/L。并得到这些环境因素对链带藻EJ12-3生长的交互影响,得到二次方程模型,该模型的拟合较好(R2为0.950),有一定的应用价值。

With the aim of isolating a high biomass accumulation strain and optimize environmental factors to enhance its biomass production,an unicellular green algae was isolated from fresh water samples,the morphological and genomic characterization identification of this strain was carried out by using 18s rRNA and ITS1 analysis.This newly isolated strain named EJ12-3 was identified as Desmodesmus sp.The environmental factors for biomass production of Desmodesmus sp.EJ12-3 was optimized by using response surface methodology(RSM).The experimental and the predicted values were very close which reflected the accuracy and the applicability of RSM(R2=0.950).According to the results,the optimal condition for biomass production within the experimental range of the variables studied was:temperature of 28℃,light intensity of 131 μmol/(m2·s),light/dark cycle of 15:9 and pH value of 6.0.Under this condition,the predicted biomass production value was up to(0.648±0.015) g/L.