硝普钠浸种对草地早熟禾种子发芽及种子内酶活性的影响

Effect of Seed Soaking with Sodium Nitroprusside on Kentucky Bluegrass Seed Germination and Enzymes Activities

为探究硝普钠(SNP)对草地早熟禾种子萌发的影响,以0,0.01,0.05,0.10,0.50,1.00,1.50 mmol/L浓度的SNP溶液分别浸泡草地早熟禾种子4,6,8,10,12 h,观察SNP对种子发芽及种子萌发过程中过氧化物酶(POD)和超氧化物歧化酶(SOD)活性的影响。结果表明,SNP浸种8 h对种子发芽具有最佳促进作用,随着浸种浓度的增大,促进种子萌发的效果表现出先升高后降低趋势,其中低浓度(0.05 mmol/L)SNP的促进效果最明显;POD和SOD酶的活性也表现出先升高后降低趋势,发芽势与2种酶的活性呈极显著正相关,而平均发芽天数与2种酶的活性呈极显著负相关。

To explore the effect of Sodium nitroprusside ( SNP ) on seeds germination of Kentucky bluegrass (KB),KB seeds were soaked with 0,0.01,0.05,0.10,0.50,1.00,1.50 mmol/L SNP for 4,6,8,10,12 h respec-tively,then seeds germination and the enzymes activities of POD and SOD were observed .The results showed that , for KB seeds germination ,the best soaking time was 8 h,and the best SNP concentration is 0.05 mmol/L.With the increased of SNP concentration ,seed germination showed increased then decreased after 0.05 mmol/L SNP.The en-zymes activities of POD and SOD were the same as seed germination .Moreover ,very significant positive correlation was found between germination energy and the two enzymes activities ,whilst significantly negative correlation was found between average germination days and the two enzymes activities .