棉花黄萎菌微菌核际拮抗细菌的筛选及其定殖能力研究

Screening and Colonization of Antagonists from Microsclerotiniasphere of Verticillium dahliae

通过微菌核诱集法从发病程度不同的棉花黄萎病田筛选大丽轮枝菌拮抗菌,测定拮抗菌对微菌核的抑制作用和在微菌核上的定殖能力。试验结果表明,从棉花黄萎菌微菌核不同部位分离的细菌数量不同,不同发病程度棉田中棉花黄萎菌拮抗菌数量不同。从微菌核内部筛选到一株能够抑制微菌核萌发和生长的拮抗菌株424-31-6,定殖能力测定结果显示,菌株424-31-6在微菌核表面定殖能力强,能够破坏微菌核的正常结构,具有防治棉花黄萎病的潜力。

Antagonists of Verticillium dahliae were screened by microsclerotia trap method from cotton fields with different damage level of Verticillium wilt.The inhibitory effect and colonization ability of the antagonists on the microsclerotia were tested .The results showed that the quantity of bacteria was different in different parts of micro-sclerotia and the quantity of antagonists varied according to different morbidity degree of Verticillium wilt in the cot-ton fields .The strain 424-31-6 which showed a good effect against germinating and growth of microsclerotia was ob-tained.It could easily colonize on the surface and destroy the normal structure of microsclerotia .The stain 424-31-6 has biocontrol potential against V.dahliae.