氯丙嗪治疗急性坏死性胰腺炎大鼠

Chlorpromazine treatment of acute necrotizing pancreatitis in rats

目的 研究磷脂酶A2(PLA2)抑制剂氯丙嗪对急性坏死性胰腺炎大鼠的治疗效果.方法 120只雌性SD大鼠按随机数字法分为对照组(30只)、ANP组(45只)和氯丙嗪组(45只).采用胰管注射5%牛磺胆酸钠溶液1 ml/kg体重制备ANP模型,对照组胰管注射等量生理盐水.氯丙嗪组于制模成功后即刻、24 h、48 h腹腔注射0.4%氯丙嗪0.25 ml/100 g体重;ANP组和对照组术后同时点腹腔注射等量生理盐水.3组于术后24 h、48 h、72 h分别处死大鼠,取血检测血淀粉酶、PLA2、IL-6;取胰腺组织行病理学检查.结果 氯丙嗪组制膜后72 h胰腺病理分值为3.57±0.73,较ANP组的13.29±1.03明显减轻.氯丙嗪组术后72 h血清淀粉酶和PLA2水平分别为(1658.0±277.0)U/L和(12.26±1.40)ng/ml,较ANP组的(3666.7±1233.0)U/L和(17.04±1.16)ng/ml显著降低(P＜0.01).氯丙嗪组术后24 h、48 h、72 h IL-6水平分别为(116.27±14.49)Pg/ml、(82.75±8.86)pg/ml、(75.35±6.17)pg/ml,也较ANP组的(160.88±27.19)Pg/ml、(125.51±30.71)pg/ml、(111.77±19.10)pg/ml显著降低(P＜0.01).结论 氯丙嗪腹腔注射治疗ANP大鼠有一定疗效.

Objective To investigate the therapeutic effect of ehlorpromazine in rats with acute necrotizing pancreatitis (ANP). Methods 120 healthy female SD rats were randomly divided into three groups: normal control group (NC group, n=30); acute necrotizing pancreatitis group (ANP group, n=45) and chlorpromazine treatment group (CPZ group, n=45). ANP was induced by the injection of 5% sodium taurocholate (1 ml/kg) into pancreatic duct, NC group were injected with same amount of saline. 0.4% chlorpromazine (0.25 ml/100 g) was intraperitoneally administered in CPZ group at 0 h, 24 h, 48 h after ANP was established. Same amount of normal saline was given to ANP group and NC group in the same way and at the same time points. The rats were sacrificed at 24, 48, 72 h after ANP was induced. The blood samples were collected for analysis of serum amylase (AMY), phospholipase A2 (PLA2), interleukin-6 (IL-6). Pancreas was harvested for evaluation of pathologic changes. Results The pathologic changes in ANP group were compatible with pathologic changes of ANP. The pathologic scores in CPZ group was 3.57±0.73 at 72 hours after ANP induced, which was significantly lower than 13.29±1.03 in ANP group. The serum amylase and PLA2 levels in CPZ group at 72 hours after ANP induced were (1658.0±277.0) U/L and ( 12.26±1.40) ng/ml respectively, the corresponding values of ANP group were (3666.7±1233.0) U/L and (16.81±1.13)ng/ml, respectively. The difference was statistically significantly (P＜0.01). The serum IL-6 levels of CPZ group at 24,48 72 hours were (116.27±14.49) pg/ml, (75.35±6.17) pg/ml, (82.75± 8.86) pg/ml respectively, the corresponding values of CPZ group were (160.88±27.19) pg/ml, (111.77± 19.10)pg/ml, (125.51±30.71) pg/ml respectively. There was a significant difference between the two groups (P＜0.01). Conclusions Chlorpromazine may have a therapeutic effect on ANP.