单管多重PCR技术快速检测五种性传播疾病的病原体研究

Rapid detection of five kinds of pathogens using single-tube multiplex PCR

目的 建立一种能同时检测淋球菌(NG)、人型支原体(MH)、生殖支原体(MG)、沙眼衣原体(CT)、解脲支原体(UU)5种病原体的单管多重PCR方法 .方法 以荧光定量PCR技术加另一种检测技术作为金标准,对单管多重PCR技术检测五种性传播疾病(STD)病原体的敏感性、特异性、准确性和重复性进行了评价.结果 该法的敏感性、特异性和符合率分别为10-9fg/μl、100%、97.8%,20份临床标本连续5 d的重复性好.结论 单管多重PCR技术为检测STD提供了一种新的方法 .

Objective To establish a multiple PCR method that can be used to spontaneously detect five kinds ofpathogens such as NG,MH,MG,CT and UU.Method With the fluorescence-quantitative PCR technique in conjunction with another detection technique as the golden standard,evaluation was conducted on the sensitivity,specificity,accuracy and repeatability on the detection of 5 kinds of STD pathogens using single-tube multiplex PCR.Result The sensitivity,specificity and match rate of the method ale 10-9fg/μl,100%,97.8%respectively,and the repeatability of 5 continuous days of 20 clinical specimens is good.Conclusion Single-tube multiplex PCR technique provides a new method to detect 5 kinds of STD pathogens.