Roscovitine对Molt-4细胞株作用的双重效应

Dual effect of Molt-4 cell lines onset with Roscovitine

目的 探讨Roscovitine作为CDK抑制剂对Molt-4细胞株作用的效应.方法 不同浓度的Roscovitine与对数生长期的Molt-4细胞共同培养不同的时间点收获,流式细胞术DNA分析R0scovitine对Molt-4细胞的抑制阻滞作用,Annexin-V/FITC法分析Roscovitine对Molt-4细胞的凋亡诱导作用.结果 Roscovitine与对数生长期的Molt-4细胞共同培养6 h后,细胞出现明显G2/M阻滞效应,18 h后这种效应达到65%以上;Roscovitine对Molt-4细胞的凋亡诱导作用与时间及浓度呈明显正相关.结论 Roscovitine对Molt-4细胞株具有双重效应,即较强的细胞阻滞作用及诱导细胞凋亡作用.

Objective To investigate the effect of Mlot-4 cells onset with Roscovitine (ROSC)as some Cyclin-dependent kinases(CDK),inhibitor.Methods The logarithmic growth phase Molt-4 cells treated with ROSC at a final concentration ranging between 1～20 μmol/L and harvested in different time point,DNA assay of single cells by flow cytometry was used to detect the effect of cell cycle arrest and Annexin-V/FITC assay was used to detect the effect of cell apoptosis. Results It showed that ROSC exerted strong inhibitory effect on proliferation and cell cycle progression of Molt-4 Accumulation of G2/M arrested cells starting 6 h after onset of 10 μmol/L and 20 μmol/L ROSC;at the same time,the cell apoptosis of Molt-4 would be detected,According with the time and concentration changing,the cell apoptosis rate would rise.Conclusion It is concluded that Roscovitine(ROSC)as some Cyclin-dependent kinases(CDK),inhibitor,It has dual effects to Molt-4 cells,not only the effect of cell cycle arrest but also the effect of cell apoptosis.