摘要
目的 研究TGase3 mRNA在食管癌中的表达情况,探讨他在食管癌发生、发展过程中的作用及意义.方法 采用组织芯片(tissue microarray,TMA)原位杂交(in situ hybridization,ISH)法检测70份食管癌组织和10份正常食管组织中TGase3 mRNA的表达情况.结果 在食管癌组织中TGase3 mRNA的失表达率为68.57%(48/70),显著高于正常对照组(P＜0.05).其表达与患者性别、年龄、肿瘤部位、淋巴结转移无关.但与临床分期、组织学分类、病理分级显著相关(P＜0.05).结论 在食管癌中存在较高频率的TGase3 mRNA失表达,TGase3基因可能参与了食管癌的发生、发展过程.
Objective To study the expression of TGase3 mRNA in esophageal carcinoma and to investigate it's role and the significance of in the carcinogenesis and progression of esophageal carcinoma.Methods The expression of TGase3 mRNA in 70 ca8es of esophageal carcinoma tissue and 10 cases of normal esophageal tissue were detected by tissue miemarray(TMA)and in situ hybridization(ISH)methods.Results The loss expression rate of TGase3mRNA in esophageal carcinoma tissue was 68.57%(48/70),which was significantly higher than in normal control(P＜0.05).The expression of TGase3 mRNA was no correlation with patients'sex,age,tumor site,lymph node metastasis.But it's significant correlation with clinical stage,histology grade,Pathology grade(P＜0.05).Conclusion There was a high frequency of TGase3 mRNA expression loss in esophageal carcinoma,TGase3 mRNA gene may be involved in carcinogenesis and progression of esophageal carcinoma.
出处
《肿瘤研究与临床》
CAS
2007年第z1期-,共4页
Cancer Research and Clinic
