具有转移能力鼻咽癌细胞株候选抗药与多药耐药相关基因的筛选

Screening potentially drug resistant and multidrug resistant genes in NPC cells with metastatic ability

目的 筛选具有转移能力的鼻咽癌细胞株中候选抗药与多药耐药相关基因.方法 利用8000点的基因芯片比较5-8F和6-10B细胞株之间的差异表达基因,并利用在线MILANO程序分析,筛选抗药与多药耐药相关基因.半定量RT-PCR验证差异表达基因.结果 分析5-8F和6-10B细胞株之间基因表达谱后,共找到283个差异表达基因,其中表达上调基因185个,下调基因98个.MILANO程序分析后,在5-8F细胞株中找到4个可能与抗药和多药耐药相关的高表达基因:UGT1A9(15.85倍)、MVP(6.77倍)、CAV1(2.49倍)和HIF1A(2.67倍).半定量RT-PCR验证4个基因筛选结果的可靠性.结论 经在线MILANO程序分析鉴定的鼻咽癌5-8F和6-10B细胞株之间的差异表达基因可能与具有转移能力鼻咽癌细胞株的抗药和多药耐药有关.

Objective To screen potential genes associated with drug resistance and multidrug resistance. Methods Microarray with 8000 genes was used to detect the different expression of 5-8F cells and 6-10B cells. Subsquently, genes of drug resistance and multidrug resistance were screened by MILANO online programme. Semiquantitative RT-PCR was utilized to confirmed the reliability of differentially expressed genes. Results 283 genes were identified the differential expression. Of these, 85 genes were shown to be upregulated and 98 downregulated. After the analysis of MILANO, 4 genes including UGT1A9 (15.85 folds),MVP(6.77 folds), CAV1(2.49 folds) and HIF1A(2.67 folds) with higher expression in 5-8F cells were found to be likely associated with drug resistance and multidrug resistance. Subsquently, semiquantitative RT-PCR confirmed the reliability of differential expression of these 4 genes. Conclusion Differentially expressed genes shown in NPC 5-8F cells compared to 6-10B cells with the identification of online MILANO program analysis are likely associated with drug resistance and mnltidrug resistance of NPC cells with the ability of metastasis.