肝癌组织中livin蛋白与突变型p53蛋白的表达及相关性研究

A study of expression and relationship of livin and mutant p53 in hepatocellular carcinoma

目的 研究livin蛋白与突变型p53蛋白在肝癌组织中的表达和意义以及探讨两者间相关性.方法 采用免疫组织化学SP法检测80例肝癌、39例肝硬化、33例肝血管瘤旁正常肝组织中livin蛋白和突变型p53蛋白的表达,并通过显微图像分析方法进行结果观察.结果 livin蛋白的表达强度依次为肝癌组织(Median=5.09;P25～P75=3.06～8.28)、肝硬化组织(Median=3.05;P25～P25=2.49～4.25)、正常肝组织(Median=1.99;P25～P75=1.54～2.54)(P<0.001);突变型p53蛋白表达强度为肝癌组织(Median=43.13;P25～P75=20.41～78.53)高于肝硬化组织(Median=20.30;P25～P75=14.90～28.08)和正常肝组织(Median=15.52;P25～P75=12.81～21.80)(P<0.001),肝硬化组织与正常肝组织间差异无统计学意义.肝癌组织中livin蛋白与突变型p53蛋向的表达呈正相关(r=0.241,P<0.05).结论 肝癌组织中livin蛋白及突变型p53蛋白的高表达以及两者呈正相关,表明两者可能对肝癌的发生与发展起着协同作用.

Objective To investigate the expression and relationship of livin and mutant p53 in hepatocellular carcinoma (HCC). Methods The expression of livin and mutant p53 were evaluated using the SP immunohistochemistry in 80 HCC tissue, 39 hepatic cirrhosis tissue, 33 normal tissue beside the hemangiomas of liver. Results The integrated A average results showed that the intension of positive expression of livin aligned by turns was HCC tissue (Median=5.09; P25～P75=3.06～8.28), hepatic cirrhosistissue(Median=3.05; P25～P75=2.49～4.25), normal liver tissue(Median=1.99; P25～P75=1.54～2.54) (P<0.001), respectively. It also showed that the intension of positive expression of p53 in HCC tissue (Median=43.13; P25～P75=20.41～78.53) was higher than that in hepatic cirrhosis tissue (Median=20.30;P25～P75=14.90～28.08), as well as in that of normal liver tissue (Median=15.52;P25～P75=12.81～21.80) (P <0.001), but it made no sense in statistics between hepatic cirrhosis tissue and normal tissue of liver. The expression of livin was obviously correlated with p53 in HCC tissue(r=0.241, P<0.05). Conclusion The overexpressian of livin and p53 and their positive correlation showed that livin may play a crucial role in the origin and development of HCC in coordination with p53.