丙戊酸钠和亚砷酸对Molt-4细胞的协同抑制作用

Synergistic antitumor effects of VPA and As2O3 on Molt-4 cells in vitro

目的 观察丙戊酸钠(VPA)以及亚砷酸(As2O3)对人类急性T细胞白血病细胞株Molt-4增生、分化和细胞周期的影响以及二者的协同作用.方法 用MTT法观察细胞生长曲线及药物对细胞增生的抑制作用,流式细胞仪检测给药前后DNA含量变化等方法,观察了As2O3和VPA对Molt-4细胞体外抗癌的协同作用.结果 VPA和As2O3均可明显抑制细胞增生,VPA对Molt-4细胞抑制率为50%的浓度即JC50卯为4.904 mmol/L;As2O3的IC50为9.925μmol/L;当二者联用时在体外有相加作用(Q值均＞0.85),在5 mmol/L VPA与10 μmol/L As2O3联用时抑制率达(70.31±2.54)%.流式细胞术检测示经不同浓度VPA处理后G1期细胞由28.21%增加至71.89%,S期细胞由71.75%减低至9.49%.结论 VPA和As2O3均可明显抑制细胞增生,两者合用有协同相加作用,其机制有待进一步研究.

Objective The study was purposed to investigate the synergistic antitumour effects of sodium valproate (VPA) and As2O3 on the proliferation, differentiation and cell cycle of Moh-4 cell in vitro.Methods Cell viability and growth curve were assessed by the MTT assay and the DNA content of Molt-4cell was analyzed by flow cytometry after exposure 36 h to VPA and As2O3. Results The results indicated that the changes of the growth curve and MTT assay showed their inhibition of proliferation to Moh-4 cells.The 50 % inhibition rate of VPA and As2O3 were 4.904 mmol/L and 9.925 μmol/L respectively. There was an addition effect with the combination of the two drugs (values of Q ＞0.85). The inhibition rate was up to (70.31±2.54)% when exposing to 5.0 mmol/L VPA combinating with 10.0 μmol/L As2O3. Flow cytometry showed that the percentage of Molt-4 ceils in G1 phase increased from 28.21% to 71.89 % and the cells in S phase decreased from 71.75 % to 9.49 % after 36 h culture of VPA. Conclusion It was found that the combinations of VPA and As2O3 for 48 h increased inhibition rate to a greater extent than occurred for cell treated with either compound alone. The mechanism needed to be further studied.